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Bright-field microscopy (BF) is the simplest of all the optical microscopy illumination techniques. Sample illumination is transmitted (i.e., illuminated from below and observed from above) white light , and contrast in the sample is caused by attenuation of the transmitted light in dense areas of the sample.
Axial bright-field detectors are located in the centre of the cone of illumination of the transmitted beam, and are often used to provide complementary images to those obtained by ADF imaging. [12] Annular bright-field detectors, located within the cone of illumination of the transmitted beam, have been used to obtain atomic resolution images ...
For bright-field microscopy, negative staining is typically performed using a black ink fluid such as nigrosin and India ink.The specimen, such as a wet bacterial culture spread on a glass slide, is mixed with the negative stain and allowed to dry.
An annular dark-field image taken may be complementary to a bright-field image constructed from the captured CBED images. The use of a hollow detector with a hole in the middle can allow for transmitted electrons to be passed to an EELS detector while scanning.
To use measurements of chlorophyll fluorescence to analyse photosynthesis, researchers must distinguish between photochemical quenching and non-photochemical quenching (heat dissipation). This is achieved by stopping photochemistry, which allows researchers to measure fluorescence in the presence of non-photochemical quenching alone.
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Microscope-based diagnostics are widely performed and served as a gold standard in histological analysis. However this procedure generally requires a series time-consuming lab-based procedures including fixation, paraffin embedment, sectioning, and staining to produce microscope slides with optically thin tissue slides (4–6 μm).
Download QR code; Print/export Download as PDF; Printable version; In other projects Wikidata item; Appearance. ... Brightfield may refer to: Bright-field microscopy;