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The design of appropriate short or long primer pairs is only one goal of PCR product prediction. Other information provided by in silico PCR tools may include determining primer location, orientation, length of each amplicon, simulation of electrophoretic mobility, identification of open reading frames, and links to other web resources. [7] [8] [9]
Viability PCR workflow. Viability PCR, also named v-PCR or vPCR, is an evolution of PCR. Through the use of a simple pre-treatment of the sample by the means of specific intercalating photo-reactive reagents it's possible to neutralize the DNA of dead cells. As a result, only DNA from live cells will be detected by PCR.
Verification and validation of computer simulation models is conducted during the development of a simulation model with the ultimate goal of producing an accurate and credible model. [ 1 ] [ 2 ] "Simulation models are increasingly being used to solve problems and to aid in decision-making.
A strip of eight PCR tubes, each containing a 100 μL reaction mixture Placing a strip of eight PCR tubes into a thermal cycler. The polymerase chain reaction (PCR) is a method widely used to make millions to billions of copies of a specific DNA sample rapidly, allowing scientists to amplify a very small sample of DNA (or a part of it) sufficiently to enable detailed study.
Live, Virtual, & Constructive (LVC) Simulation is a broadly used taxonomy for classifying Modeling and Simulation (M&S). However, categorizing a simulation as a live, virtual, or constructive environment is problematic since there is no clear division among these categories. The degree of human participation in a simulation is infinitely ...
Recombinase polymerase amplification (RPA) is a single tube, isothermal alternative to the polymerase chain reaction (PCR). [1] By adding a reverse transcriptase enzyme to an RPA reaction, it can detect RNA as well as DNA, without the need for a separate step to produce cDNA.
In the field of computational neuroscience, brain simulation is the concept of creating a functioning computer model of a brain or part of a brain. [1] Brain simulation projects intend to contribute to a complete understanding of the brain, and eventually also assist the process of treating and diagnosing brain diseases .
A controlled payment number, disposable credit card or virtual credit card is an alias for a credit card number, with a limited number of transactions, and an expiration date between two and twelve months from the issue date. This "alias" number is indistinguishable from an ordinary credit card number, and the user's actual credit card number ...