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  2. Genome editing - Wikipedia

    en.wikipedia.org/wiki/Genome_editing

    Genome editing, or genome engineering, or gene editing, is a type of genetic engineering in which DNA is inserted, deleted, modified or replaced in the genome of a living organism. Unlike early genetic engineering techniques that randomly insert genetic material into a host genome, genome editing targets the insertions to site-specific locations.

  3. Gene targeting - Wikipedia

    en.wikipedia.org/wiki/Gene_targeting

    The DSB is then repaired through two alternative DNA repair pathways (NHEJ or HR) to lead to random mutations at the cut site ("targeted mutagenesis") or specific mutations if a repair template is supplied that contains those specific edits ("gene targeting"). The two most established forms of gene editing are gene-targeting and targeted ...

  4. CRISPR gene editing - Wikipedia

    en.wikipedia.org/wiki/CRISPR_gene_editing

    Whereas gene editing involves changing the actual DNA sequence itself, epigenetic editing involves modifying and presenting DNA sequences to proteins and other DNA binding factors that influence DNA function. By "editing” epigenomic features in this manner, researchers can determine the exact biological role of an epigenetic modification at ...

  5. Genetic engineering techniques - Wikipedia

    en.wikipedia.org/wiki/Genetic_engineering_techniques

    All genetic engineering processes involve the modification of DNA. Traditionally DNA was isolated from the cells of organisms. Later, genes came to be cloned from a DNA segment after the creation of a DNA library or artificially synthesised. Once isolated, additional genetic elements are added to the gene to allow it to be expressed in the host ...

  6. CRISPR - Wikipedia

    en.wikipedia.org/wiki/CRISPR

    CRISPR gene editing is a revolutionary technology that allows for precise, targeted modifications to the DNA of living organisms. Developed from a natural defense mechanism found in bacteria, CRISPR-Cas9 is the most commonly used system.

  7. Genetic engineering - Wikipedia

    en.wikipedia.org/wiki/Genetic_engineering

    Genetic engineering, also called genetic modification or genetic manipulation, is the modification and manipulation of an organism's genes using technology.It is a set of technologies used to change the genetic makeup of cells, including the transfer of genes within and across species boundaries to produce improved or novel organisms.

  8. Gene editing - Wikipedia

    en.wikipedia.org/wiki/Gene_editing

    Gene editing may refer to: . Genetic engineering of any organism by genome editing. Gene editing is the emerging molecular biology technique which makes very specific targeted changes by insertion, deletion or substitution of genetic material in an organism's DNA to obtain desired results.

  9. Recombinant AAV mediated genome engineering - Wikipedia

    en.wikipedia.org/wiki/Recombinant_AAV_mediated...

    The technique builds on Mario Capecchi and Oliver Smithies' Nobel Prize–winning discovery that homologous recombination (HR), a natural hi-fidelity DNA repair mechanism, can be harnessed to perform precise genome alterations in mice. rAAV mediated genome-editing improves the efficiency of this technique to permit genome engineering in any pre ...