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It is used to stain collagen. If blue is preferred to green, methyl blue or water blue can be substituted. Standard applications: Masson's trichrome staining is widely used to study muscular pathologies (muscular dystrophy), cardiac pathologies , hepatic pathologies or kidney pathologies (glomerular fibrosis). It can also be used to detect and ...
In "one-step" methods, all the dyes—with or without a polyacid—are combined in a single solution. One of the oldest single-step approaches to trichrome staining is van Gieson's method, which stains muscle and cytoplasm yellow, and collagen red. Another is the Gömöri trichrome stain, which closely mimics Masson's trichrome. In "yellowsolve ...
Phosphomolybdic acid is the heteropolymetalate with the formula H 3 [Mo 12 PO 40]·12H 2 O. It is a yellow solid, although even slightly impure samples have a greenish coloration. It is also known as dodeca molybdophosphoric acid or PMA, is a yellow-green chemical compound that is freely soluble in water and polar organic solvents such as ethanol.
Ponceau 2R, Xylidine ponceau, Ponceau G, Red R, Acid Red 26, Food Red 5, or C.I. 16150 is a red azo dye used in histology for staining.It is easily soluble in water and slightly in ethanol.
Masson's trichrome is (as the name implies) a three-colour staining protocol. The recipe has evolved from Masson's original technique for different specific applications, but all are well-suited to distinguish cells from surrounding connective tissue .
In Masson's trichrome, it is used as a counterstain to acid fuchsin. It is a component of Papanicolaou stains together with eosin Y and bismarck brown Y. [1] In pap smears, Light Green SF confers a blue staining for the cytoplasm of active cells such as columnar cells, parabasal squamous cells, and intermediate squamous cells. [3]
Acid fuchsin or fuchsine acid, (also called Acid Violet 19 [1] and C.I. 42685 [1]) is an acidic magenta dye with the chemical formula C 20 H 17 N 3 Na 2 O 9 S 3. It is a sodium sulfonate derivative of fuchsine. Acid fuchsin has wide use in histology, [1] and is one of the dyes used in Masson's trichrome stain. [2]
Aniline blue or its constituents are used to stain collagen, as the fibre stain in Masson's trichrome, [5] as well as to reveal callose structures in plant tissues. [6] It can also be used in other connective tissue stains, such as Mallory's stain, [5] Gömöri trichrome stain, and Carstair's Method. [7] It is used in differential staining.