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It is used to stain collagen. If blue is preferred to green, methyl blue or water blue can be substituted. Standard applications: Masson's trichrome staining is widely used to study muscular pathologies (muscular dystrophy), cardiac pathologies , hepatic pathologies or kidney pathologies (glomerular fibrosis). It can also be used to detect and ...
The first staining protocol that was described as "trichrome" was Mallory's trichrome stain, which differentially stained erythrocytes to a red colour, muscle tissue to a red colour, and collagen to a blue colour. Some other trichrome staining protocols are the Masson's trichrome stain, Lillie's trichrome, and the Gömöri trichrome stain.
Phosphomolybdic is used as a stain for developing thin-layer chromatography plates, [4] staining phenolics, hydrocarbon waxes, alkaloids, and steroids. Conjugated unsaturated compounds reduce PMA to molybdenum blue. The color intensifies with increasing number of double bonds in the molecule being stained. [5]
Lillie's trichrome is a combination of dyes used in histology. It is similar to Masson's trichrome stain , but it uses Biebrich scarlet for the plasma stain. It was initially published by Ralph D. Lillie in 1940. [ 1 ]
In Masson's trichrome, it is used as a counterstain to acid fuchsin. It is a component of Papanicolaou stains together with eosin Y and bismarck brown Y. [1] In pap smears, Light Green SF confers a blue staining for the cytoplasm of active cells such as columnar cells, parabasal squamous cells, and intermediate squamous cells. [3]
Acid fuchsin has wide use in histology, [1] and is one of the dyes used in Masson's trichrome stain. [2] This method is commonly used to stain cytoplasm and nuclei of tissue sections in the histology laboratory in order to distinguish muscle from collagen .
Aniline blue or its constituents are used to stain collagen, as the fibre stain in Masson's trichrome, [5] as well as to reveal callose structures in plant tissues. [6] It can also be used in other connective tissue stains, such as Mallory's stain, [5] Gömöri trichrome stain, and Carstair's Method. [7] It is used in differential staining.
Download as PDF; Printable version; ... Trichrome may refer to: Trichrome staining, a histological staining method; Trichromacy, ...