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Ammonium sulfate is an inorganic salt with a high solubility that disassociates into ammonium (NH + 4) and sulfate (SO 2− 4) in aqueous solutions. [1] Ammonium sulfate is especially useful as a precipitant because it is highly soluble, stabilizes protein structure, has a relatively low density, is readily available, and is relatively inexpensive.
Salting out (also known as salt-induced precipitation, salt fractionation, anti-solvent crystallization, precipitation crystallization, or drowning out) [1] is a purification technique that utilizes the reduced solubility of certain molecules in a solution of very high ionic strength.
Calcium carbonate precipitates as a solid, leaving ammonium sulfate in the solution. (NH 4) 2 CO 3 + CaSO 4 → (NH 4) 2 SO 4 + CaCO 3. Ammonium sulfate occurs naturally as the rare mineral mascagnite in volcanic fumaroles and due to coal fires on some dumps. [14] Ammonium sulfate is a byproduct in the production of methyl methacrylate. [15]
The following chart shows the solubility of various ionic compounds in water at 1 atm pressure and room temperature (approx. 25 °C, 298.15 K). "Soluble" means the ionic compound doesn't precipitate, while "slightly soluble" and "insoluble" mean that a solid will precipitate; "slightly soluble" compounds like calcium sulfate may require heat to precipitate.
Substance Formula 0 °C 10 °C 20 °C 30 °C 40 °C 50 °C 60 °C 70 °C 80 °C 90 °C 100 °C Barium acetate: Ba(C 2 H 3 O 2) 2: 58.8: 62: 72: 75: 78.5: 77: 75
Also used in cosmetics and skin care products. Azodicarbonamide – flour bleaching agent. Also used in the production of foamed plastics and the manufacture of gaskets. Banned as a food additive in Australia and Europe. Azorubine – color (red) (FDA: Ext D&C Red #10)
ABC dry chemical is usually a mix of monoammonium phosphate and ammonium sulfate, the former being the active component. The mix between the two agents is usually 40–60%, 60–40%, or 90–10% depending on local standards worldwide. The USGS uses a similar mixture, called Phos Chek G75F. [2]
NaOH reacts the ammonium (NH 4 +) to ammonia (NH 3), which boils off the sample solution. Ammonia bubbles through the standard acid solution and reacts back to ammonium salts with the weak or strong acid. [3] Ammonium ion concentration in the acid solution, and thus the amount of nitrogen in the sample, is measured via titration.