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The starch iodine test, a development of the iodine test, is based on colour change, as α-amylase degrades starch and is commonly used in many applications. A similar but industrially produced test is the Phadebas amylase test, which is used as a qualitative and quantitative test within many industries, such as detergents, various flour, grain ...
The Phadebas Forensic Press test is used as a presumptive test for saliva. [5] Phadebas may be used to find saliva as a DNA source, or to identify the origin of a stain. The test is performed by placing paper bound with the Phadebas substrate to a sample, and applying pressure. [5]
A common protocol used in the past for zymography of α-amylase activity was the so-called starch film protocol of W.W. Doane. Here a native PAGE gel was run to separate the proteins in a homogenate. Subsequently, a thin gel with starch dissolved (or more properly, suspended) in it was overlaid for a period of time on top of the original gel. [6]
An anti-α-amylase antibody is fixed at the test line. If human salivary amylase is present in the sample, it will form an antigen-antibody-colloidal gold complex upon deposition. These complexes react with the antibodies fixed at the test line to form a visible red line indicating the presence of human salivary α-amylase, and therefore, human ...
The procedure for Bradford protein assay is very easy and simple to follow. It is done in one step where the Bradford reagent is added to a test tube along with the sample. After mixing well, the mixture almost immediately changes to a blue color.
A presumptive test to detect saliva is the alpha-amylase test also known as the Phadebas Test. [4] This detection technique is based on the activity of the enzyme alpha-amylase which breaks down starches from food into smaller oligosaccharide molecules, starting digestion in the mouth. [11]