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  2. Protein–DNA interaction site predictor - Wikipedia

    en.wikipedia.org/wiki/ProteinDNA_interaction...

    The amount of predicted features is much larger than of DISIS (previous version). Finally, DISIS2 is able to predict DNA-binding residues from protein sequence of DNA-binding proteins. DNABindR predicts DNA binding sites from amino acid sequences using machine learning algorithms. [3] DISPLAR makes a prediction based on properties of protein ...

  3. Two-hybrid screening - Wikipedia

    en.wikipedia.org/wiki/Two-hybrid_screening

    Two-hybrid screening (originally known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover proteinprotein interactions (PPIs) [1] and proteinDNA interactions [2] [3] by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.

  4. Methods to investigate protein–protein interactions - Wikipedia

    en.wikipedia.org/wiki/Methods_to_investigate...

    Proteinprotein docking, the prediction of proteinprotein interactions based only on the three-dimensional protein structures from X-ray diffraction of protein crystals might not be satisfactory. [44] [45] Network analysis includes the analysis of interaction networks using methods of graph theory or statistical methods.

  5. Functional genomics - Wikipedia

    en.wikipedia.org/wiki/Functional_genomics

    A yeast two-hybrid screening (Y2H) tests a "bait" protein against many potential interacting proteins ("prey") to identify physical proteinprotein interactions. This system is based on a transcription factor, originally GAL4, [9] whose separate DNA-binding and transcription activation domains are both required in order for the protein to ...

  6. Proximity ligation assay - Wikipedia

    en.wikipedia.org/wiki/Proximity_ligation_assay

    Figure 1: PLA starts with the binding of antibodies from different species to 2 proteins of interest, in this case protein * (star) and protein#. Proximity ligation assay (in situ PLA) is a technology that extends the capabilities of traditional immunoassays to include direct detection of proteins, protein interactions, extracellular vesicles and post translational modifications with high ...

  7. Chromatin immunoprecipitation - Wikipedia

    en.wikipedia.org/wiki/Chromatin_immunoprecipitation

    Cross-linked DNA fragments associated with the protein(s) of interest are selectively immunoprecipitated from the cell debris using an appropriate protein-specific antibody. The associated DNA fragments are purified and their sequence is determined. Enrichment of specific DNA sequences represents regions on the genome that the protein of ...

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