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PSF Lab is a software program that allows the calculation of the illumination point spread function (PSF) of a confocal microscope under various imaging conditions. The calculation of the electric field vectors is based on a rigorous, vectorial model that takes polarization effects in the near-focus region and high numerical aperture microscope objectives into account.
Amira (ah-MEER-ah) is a software platform for visualization, processing, and analysis of 3D and 4D data. It is being actively developed by Thermo Fisher Scientific in collaboration with the Zuse Institute Berlin (ZIB), and commercially distributed by Thermo Fisher Scientific — together with its sister software Avizo.
Fluorescence and confocal microscopes operating principle. Confocal microscopy, most frequently confocal laser scanning microscopy (CLSM) or laser scanning confocal microscopy (LSCM), is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out-of-focus light in image formation. [1]
The point spread function (PSF) describes the response of a focused optical imaging system to a point source or point object. A more general term for the PSF is the system's impulse response; the PSF is the impulse response or impulse response function (IRF) of a focused optical imaging system. The PSF in many contexts can be thought of as the ...
The optical transfer function of a well-focused (a), and an out-of-focus optical imaging system without aberrations (d). As the optical transfer function of these systems is real and non-negative, the optical transfer function is by definition equal to the modulation transfer function (MTF).
OpenCV (Open Source Computer Vision Library) is a library of programming functions mainly for real-time computer vision. [2] Originally developed by Intel, it was later supported by Willow Garage, then Itseez (which was later acquired by Intel [3]). The library is cross-platform and licensed as free and open-source software under Apache License ...
Often digital cameras used for this application provide pixel intensity data to a resolution of 12-16 bits, much higher than is used in consumer imaging products. Ironically, in recent years, much effort has been put into acquiring data at video rates, or higher (25-30 frames per second or higher). What was once easy with off-the-shelf video ...
Confocal stacks will require 3D processing and widefield pseudo-stacks will often benefit from digital deconvolution to remove the out-of-focus light. The advent of automated microscopes that can acquire many images automatically is one of the reasons why analysis cannot be done by eye (otherwise, annotation would rapidly become the research ...