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Though ULM achieves resolutions under 10 micrometers, "motion in the body or from the ultrasound transducer can be several orders of magnitudes beyond this level". [3] [11] For example, body motion could be on the scale of 0.5 mm, while ULM resolutions may be 5 to 10 micrometers. In addition, ULM requires "many localizations over many frames ...
Ultrasound Doppler imaging can be used to obtain basic functional measurements of brain activity using blood flow. In functional transcranial Doppler sonography, a low frequency (1-3 MHz) transducer is used through the temporal bone window with a conventional pulse Doppler mode to estimate blood flow at a single focal location.
English: The main applications and features of functional ultrasound (fUS) imaging. fUS imaging provides (i) a compatibility with a wide range of animal models for preclinical studies, (ii) the ability to image awake and freely moving animals, (iii) possibility to combine with super-resolution ultrasound localization microscopy, (iv) possible extension to 3D imaging, (v) functional ...
A dual detector head gamma camera combined with a CT scanner, which provides localization of functional SPECT data, is termed a SPECT-CT camera, and has shown utility in advancing the field of molecular imaging. In most other medical imaging modalities, energy is passed through the body and the reaction or result is read by detectors.
Multi-spectral. MSOT collects images at multiple wavelengths and resolves the spectral signatures in each voxel imaged, making it a multi-spectral method. Typically, MSOT is used to generate three images: one anatomical image at a single wavelength, one functional image resolving oxy- and deoxy-hemoglobin concentrations, and a third image resolving additional target photoabsorber(s).
In comparison to other super-resolution microscopy techniques such as STORM or PALM that rely on single-molecule localization and hence only allow one active molecule per diffraction-limited area (DLA) and timepoint, [1] [2] SOFI does not necessitate a controlled photoswitching and/ or photoactivation as well as long imaging times.
Photo-activated localization microscopy (PALM or FPALM) [1] [2] and stochastic optical reconstruction microscopy (STORM) [3] are widefield (as opposed to point scanning techniques such as laser scanning confocal microscopy) fluorescence microscopy imaging methods that allow obtaining images with a resolution beyond the diffraction limit.
The most common example of molecular imaging used clinically today is to inject a contrast agent (e.g., a microbubble, metal ion, or radioactive isotope) into a patient's bloodstream and to use an imaging modality (e.g., ultrasound, MRI, CT, PET) to track its movement in the body.