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Oil immersion objective lenses look superficially identical to non-oil immersion lenses. In light microscopy , oil immersion is a technique used to increase the resolving power of a microscope . This is achieved by immersing both the objective lens and the specimen in a transparent oil of high refractive index , thereby increasing the numerical ...
However, for most applications it is recommended that oil immersion be used with fixed (dead) specimens because live cells require an aqueous environment, and the mixing of oil and water can cause severe spherical aberrations. For some applications silicone oil can be used to produce more accurate image reconstructions. Silicone oil is an ...
Immersion oil has the same refraction as glass and improves the resolution of the observed specimen. Use of sample-staining methods for use in microbiology , such as simple stains ( methylene blue , safranin , crystal violet ) and differential stains (negative stains, flagellar stains, endospore stains).
An oil immersion objective is an objective lens specially designed to be used in this way. The index of the oil is typically chosen to match the index of the microscope lens glass, and of the cover slip. For more details, see the main article, oil immersion. Some microscopes also use other index-matching materials besides oil; see water ...
Some microscopes make use of oil-immersion objectives or water-immersion objectives for greater resolution at high magnification. These are used with index-matching material such as immersion oil or water and a matched cover slip between the objective lens and the sample. The refractive index of the index-matching material is higher than air ...
"About 85-90 percent [of swimmers] were finding some benefit from it," he says. Other research suggests cold water immersion can reduce insulin resistance and help treat chronic autoimmune ...
As with objective lenses, a condenser lens with a maximum numerical aperture of greater than 0.95 is designed to be used under oil immersion (or, more rarely, under water immersion), with a layer of immersion oil placed in contact with both the slide/coverslip and the lens of the condenser. An oil immersion condenser may typically have NA of up ...
Seymour R. Cray Jr. founder of Cray Research, LLC patented a "Immersion cooled high density electronic assembly" in 1982. [23] The Cray T90 (released in 1995) used large liquid-to-chilled-liquid heat exchangers and single or two-phase immersion cooling liquids for heat removal [24]