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India ink staining, also known as negative staining, is a simple yet effective approach for visualizing the exterior structures of bacteria, fungi, and other microbes in microbiology. The dye is used to stain the background in this staining process, while the microbe shows as a clear patch against the dark background.
Procedure of India Ink Preparation. Put on gloves. Centrifuge the CSF for 5 to 10 minutes. Remove the supernatant fluid and mix the sediment. Transfer an equal amount of sediment and India ink i.e. a drop of the sediment to a slide and add a drop of India ink. Mix and cover with a coverslip.
The India ink test is a quick procedure that can provide valuable information in diagnosing Cryptococcus infections. By following these simple steps, clinicians can accurately detect Cryptococcus cells in CSF.
One very simple approach is mixing cells in a preparation of India ink. The large particles of ink will not penetrate the tight layers of the capsule or stain the bacterium. The particles of the ink will however provide a negative background that allows visualization of cells and capsules.
Cryptococcus neoformans, because of its large polysaccharide capsule, can be visualized by the India Ink stain. Organisms that possess a polysaccharide capsule exhibit a halo around the cell against the black background created by the India Ink.
Demonstration of the proper technique for performing an India ink stain.This video can also be viewed athttps://reach.cdc.gov/job-aids-resources.
Principle of Negative Staining. Negative staining requires an acidic dye such as India Ink or Nigrosin. India Ink or Nigrosin is an acidic stain. This means that the stain readily gives up a hydrogen ion (proton) and the chromophore of the dye becomes negatively charged.
Are you curious about India ink staining and how it is used in microbiology? Look no further! we will explore the principle, procedure, and results of India ...
PRINCIPLE AND RESULTS: This stain is intended for use by laboratory professionals to stain (in vitro) Cryptococcus neoformans in cerebral spinal fluid, which will appear as encapsulated cells that appear to have a clear, unstained zone surrounding the cell.
Introduction. The Diagnosis of Cryptococcal Meningitis. Cryptococcal Antigen Testing (CrAg) Latex Agglutination (LA) and Enzyme-Linked ImmunoSorbert Assays (ELISA) Lateral Flow Assay. India Ink Staining. CrAg Testing for the Prevention of Cryptococcal Meningitis. Logistical Challenges of CrAg Screening. Non-Meningeal Cryptoccal Disease.