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Numerical aperture of a thin lens. Numerical aperture is not typically used in photography. Instead, the angular aperture of a lens (or an imaging mirror) is expressed by the f-number, written f /N, where N is the f-number given by the ratio of the focal length f to the diameter of the entrance pupil D: =.
A 100 mm focal length f /4 lens has an entrance pupil diameter of 25 mm. A 100 mm focal length f /2 lens has an entrance pupil diameter of 50 mm. Since the area is proportional to the square of the pupil diameter, [6] the amount of light admitted by the f /2 lens is four times that of the f /4 lens.
The f-number ("relative aperture"), N, is defined by N = f / E N, where f is the focal length and E N is the diameter of the entrance pupil. [2] Increasing the focal length of a lens (i.e., zooming in) will usually cause the f-number to increase, and the entrance pupil location to move further back along the optical axis.
A more typical consumer zoom will have a variable maximum relative aperture since it is harder and more expensive to keep the maximum relative aperture proportional to the focal length at long focal lengths; f /3.5 to f /5.6 is an example of a common variable aperture range in a consumer zoom lens.
Here NA is the numerical aperture, is half the included angle of the lens, which depends on the diameter of the lens and its focal length, is the refractive index of the medium between the lens and the specimen, and is the wavelength of light illuminating or emanating from (in the case of fluorescence microscopy) the sample.
The angular aperture of a thin lens with focal point at F and an aperture of diameter ... is the focal length ... the numerical aperture in air is:
The eye includes a lens similar to lenses found in optical instruments such as cameras and the same physics principles can be applied. The pupil of the human eye is its aperture; the iris is the diaphragm that serves as the aperture stop.
The pupil function or aperture function describes how a light wave is affected upon transmission through an optical imaging system such as a camera, microscope, or the human eye. More specifically, it is a complex function of the position in the pupil [ 1 ] or aperture (often an iris ) that indicates the relative change in amplitude and phase ...