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  2. Tac-Promoter - Wikipedia

    en.wikipedia.org/wiki/Tac-Promoter

    The tac promoter consists of the '–35' region of the trp promoter and the '–10' region of the lac promoter (and differs from a related trc promoter by 1 bp [3]). The tac promoter is, therefore, inducible by IPTG (Isopropyl β- D -1-thiogalactopyranoside), whilst also allowing higher maximum gene expression than either the lac or trp promoters.

  3. Tetracycline-controlled transcriptional activation - Wikipedia

    en.wikipedia.org/wiki/Tetracycline-controlled...

    Example of a T-REx system controlling the expression of shRNA. Tetracycline-controlled transcriptional activation is a method of inducible gene expression where transcription is reversibly turned on or off in the presence of the antibiotic tetracycline or one of its derivatives (e.g. doxycycline).

  4. General transcription factor - Wikipedia

    en.wikipedia.org/wiki/General_transcription_factor

    A transcription factor is a protein that binds to specific DNA sequences (enhancer or promoter), either alone or with other proteins in a complex, to control the rate of transcription of genetic information from DNA to messenger RNA by promoting (serving as an activator) or blocking (serving as a repressor) the recruitment of RNA polymerase.

  5. Promoter (genetics) - Wikipedia

    en.wikipedia.org/wiki/Promoter_(genetics)

    As promoters are typically immediately adjacent to the gene in question, positions in the promoter are designated relative to the transcriptional start site, where transcription of DNA begins for a particular gene (i.e., positions upstream are negative numbers counting back from -1, for example -100 is a position 100 base pairs upstream).

  6. Expression vector - Wikipedia

    en.wikipedia.org/wiki/Expression_vector

    The promoters used for these vector are usually based on the promoter of the lac operon or the T7 promoter, [11] and they are normally regulated by the lac operator. These promoters may also be hybrids of different promoters, for example, the Tac-Promoter is a hybrid of trp and lac promoters. [12]

  7. Grammatical particle - Wikipedia

    en.wikipedia.org/wiki/Grammatical_particle

    In modern grammar, a particle is a function word that must be associated with another word or phrase to impart meaning, i.e., it does not have its own lexical definition. [citation needed] According to this definition, particles are a separate part of speech and are distinct from other classes of function words, such as articles, prepositions, conjunctions and adverbs.

  8. Grammatical category - Wikipedia

    en.wikipedia.org/wiki/Grammatical_category

    A phonological manifestation of a category value (for example, a word ending that marks "number" on a noun) is sometimes called an exponent. Grammatical relations define relationships between words and phrases with certain parts of speech, depending on their position in the syntactic tree.

  9. Topicalization - Wikipedia

    en.wikipedia.org/wiki/Topicalization

    Assuming that the a-sentences represent canonical word order, the b-sentences contain instances of topicalization. The constituent in bold is fronted to establish it as topic. The first two examples, which use topicalized adjuncts, are typical, but the last two examples with topicalized object arguments are comparatively rare.