Search results
Results From The WOW.Com Content Network
Unlike high-content analysis, high-content screening implies a level of throughput which is why the term "screening" differentiates HCS from HCA, which may be high in content but low in throughput. In high content screening, cells are first incubated with the substance and after a period of time, structures and molecular components of the cells ...
High-throughput screening (HTS) is a method for scientific discovery especially used in drug discovery and relevant to the fields of biology, materials science [1] and chemistry. [ 2 ] [ 3 ] Using robotics , data processing/control software, liquid handling devices, and sensitive detectors, high-throughput screening allows a researcher to ...
Some examples are: high-throughput and high-fidelity quantification and sub-cellular localization (high-content screening, cytohistopathology, Bioimage informatics) morphometrics; clinical image analysis and visualization; determining the real-time air-flow patterns in breathing lungs of living animals
Classical High throughput screening robotics are now being tied closer to cell biology, principally using technologies such as High-content screening.High throughput cell biology dictates methods that can take routine cell biology from low scale research to the speed and scale necessary to investigate complex systems, achieve high sample size, or efficiently screen through a collection.
High-content screening where changes in the expression of several proteins can be simultaneously monitored is also often used. [ 9 ] [ 10 ] High-content imaging of dye-labeled cellular components can also reveal effects of compounds on cell cultures in vitro, distinguishing the phenotypic effects of a broad variety of drugs.
For example, if σ p =σ n =1, then μ p =6 and μ n =0 gives a zero Z-factor. But for normally-distributed data with these parameters, the probability that the positive control value would be less than the negative control value is less than 1 in 10 5. Extreme conservatism is used in high throughput screening due to the large number of tests ...
After hits are identified from a high throughput screen, the hits are confirmed and evaluated using the following methods: Confirmatory testing: compounds that were found active against the selected target are re-tested using the same assay conditions used during the HTS to make sure that the activity is reproducible.
High Resolution Melt (HRM) analysis is a powerful technique in molecular biology for the detection of mutations, polymorphisms and epigenetic differences in double-stranded DNA samples. It was discovered and developed by Idaho Technology and the University of Utah. [ 1 ]