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Single-molecule real-time (SMRT) sequencing is a parallelized single molecule DNA sequencing method. Single-molecule real-time sequencing utilizes a zero-mode waveguide (ZMW). [1] A single DNA polymerase enzyme is affixed at the bottom of a ZMW with a single molecule of DNA as a template.
The company's first scientific instrument, called the PacBio RS, was released to a limited set of customers in late 2010., with full commercial release in early 2011. [17] [8] Sequencing provider GATC Biotech was selected by Pacific Biosciences as its first European service provider in late 2010. [18]
PacBio's (PACB) sequencing system enhances Radboudumc's SMRT sequencing capability through the addition of two new PacBio Sequel IIe systems.
Pacific Biosciences produces the PacBio RS and Sequel sequencing systems using a single molecule real time sequencing, or SMRT, method. [31] This system can produce read lengths of multiple thousands of base pairs.
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New methods such as next-generation sequencing (NGS) and single-molecule real-time (SMRT) sequencing have enabled faster, more accurate, and more cost-effective sequencing of RNA molecules. These advances have opened up new possibilities for studying gene expression, identifying new genes, and understanding the regulation of gene expression.