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Contamination on an agar plate. An agar plate is a Petri dish that contains a growth medium solidified with agar, used to culture microorganisms. Sometimes selective compounds are added to influence growth, such as antibiotics. [1] 96 pinner used to perform spot assays with yeast, fungal or bacterial cells
The molten agar is cooled to about 45 degrees Celsius and is poured using a sterile method into a petri dish containing a specific diluted sample. From here, the plates are rotated to ensure the samples are uniformly mixing with the agar. Incubation of the plates is the next step and is carried out for about 3 days at 20 to 30 degrees Celsius.
The Petri dish was developed by German physician Julius Richard Petri (after whom the name is given) while working as an assistant to Robert Koch at Berlin University.Petri did not invent the culture dish himself; rather, it was a modified version of Koch's invention [9] which used an agar medium that was developed by Walther Hesse. [10]
Microtitre plates: mostly used for ELISA: Microtome: cuts prepared specimens for analysis under microscope Nichrome wire loop: used to inoculate test samples into culture media for bacterial or fungal cultures, antibiograms, etc.; sterilized by flaming to red hot before use Petri dish/agar plate: to act as a supporting container to hold the ...
Agar plates Organisms are placed or streaked onto petri dishes Provides a solid surface for stationary growth, compact and stackable Agar based dipsticks Essentially miniature agar plates in the form of dipsticks Diagnostic purposes, can be used anywhere, cost effective, easy to use Selective and differential media
The media used in Kirby–Bauer testing must be Mueller–Hinton agar at only 4 mm deep, poured into either 100 mm or 150 mm Petri dishes. The pH level of the agar must be between 7.2 and 7.4. Bacterial inoculum is prepared by diluting a broth culture to match a 0.5 McFarland turbidity standard , which is equivalent to approximately 150 million ...