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Add two nitrate tablets to the sample. If the bacterium produces nitrate reductase, the broth will turn a deep red within 5 minutes at this step. If no color change is observed, then the result is inconclusive. Add a small amount of zinc to the broth. If the solution remains colorless, then both nitrate reductase and nitrite reductase are ...
Experiments and Observations on Different Kinds of Air (1774–86) is a six-volume work published by 18th-century British polymath Joseph Priestley which reports a series of his experiments on "airs" or gases, most notably his discovery of the oxygen gas (which he called "dephlogisticated air").
Respirometry depends on a "what goes in must come out" principle. [6] Consider a closed system first. Imagine that we place a mouse into an air-tight container. The air sealed in the container initially contains the same composition and proportions of gases that were present in the room: 20.95% O 2, 0.04% CO 2, water vapor (the exact amount depends on air temperature, see dew point), 78% ...
Functional residual capacity (FRC) cannot be measured via spirometry, but it can be measured with a plethysmograph or dilution tests (for example, helium dilution test). Average values for forced vital capacity (FVC), forced expiratory volume in 1 second (FEV1) and forced expiratory flow 25–75% (FEF25–75%), according to a study in the ...
The amount of He in the spirometer is known at the beginning of the test (concentration × volume = amount). The patient is then asked to breathe (normal breaths) in the mixture starting from FRC (functional residual capacity), which is the gas volume in the lung after a normal breath out. The spirometer measures helium concentration.
Although physiologic respiration is necessary to sustain cellular respiration and thus life in animals, the processes are distinct: cellular respiration takes place in individual cells of the organism, while physiologic respiration concerns the diffusion and transport of metabolites between the organism and the external environment.
Cheyne–Stokes respiration is a breathing pattern consisting of alternating periods of rapid and slow breathing, which may result from a brain stem injury. [12] Cheyne-Stokes respiration may be observed in newborn babies, but this is occasionally physiological (normal). Chest retractions may be observed in patients with asthma.
Huff and Puff Apparatus respiration demonstration. The huff and puff apparatus is used in school biology labs to demonstrate that carbon dioxide is a product of respiration. A pupil breathes in and out of the middle tube. The glass tubing is arranged in such a way that one flask bubbles as the pupils breathes in, the other as the pupil breathes ...