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The strict regulation of translation in both space and time is in part governed by cis-regulatory elements located in 5′ mRNA transcript leaders (TLs) and 3′ untranslated regions (UTRs). Due to their role in translation initiation, mRNA 5′ transcript leaders (TLs) strongly influence protein expression.
SILAC has emerged as a very powerful method to study cell signaling, post translation modifications such as phosphorylation, [6] [7] protein–protein interaction and regulation of gene expression. In addition, SILAC has become an important method in secretomics, the global study of secreted proteins and secretory pathways. [8]
some of the protein complexes involved in initiation. Initiation of translation usually involves the interaction of certain key proteins, the initiation factors, with a special tag bound to the 5'-end of an mRNA molecule, the 5' cap, as well as with the 5' UTR. These proteins bind the small (40S) ribosomal subunit and hold the mRNA in place. [1]
The process of amino acid building to create protein in translation is a subject of various physic models for a long time starting from the first detailed kinetic models such as [26] or others taking into account stochastic aspects of translation and using computer simulations. Many chemical kinetics-based models of protein synthesis have been ...
A food safety hazard is any biological, chemical, or physical property that may cause a food to be unsafe for human consumption. Identify critical control points A critical control point (CCP) is a point, step, or procedure in a food manufacturing process at which control can be applied and, as a result, a food safety hazard can be prevented ...
Protein detection technique has been utilized to discover protein in different category food, such as soybean (bean), walnut (nut), and beef (meat). [4] Protein detection method for different type food vary on the basis of property of food for bean, nut and meat. Protein detection has different application in different field.
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The eIF2 alpha subunit is characterized by an OB-fold domain and two beta strands. This subunit helps to regulate translation, as it becomes phosphorylated to inhibit protein synthesis. [2] The eIF4F complex supports the cap-dependent translation initiation process and is composed of the initiation factors eIF4A, eIF4E, and eIF4G.
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