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Ethylenediaminetetraacetic acid (EDTA), also called EDTA acid, is an aminopolycarboxylic acid with the formula [CH 2 N(CH 2 CO 2 H) 2] 2. This white, slightly water-soluble solid is widely used to bind to iron (Fe 2+ /Fe 3+) and calcium ions (Ca 2+), forming water-soluble complexes even at neutral pH. It is thus used to dissolve Fe- and Ca ...
structure of solid sodiium molybdate dihydrate. In aqueous solution, sodium molybdate features dissociated sodium ions and tetrahedral molybdate (MoO 4 2-), which adopts a sulfate-like structure. The solid dihydrate material has a complex structure typical for alkali metal salts of oxyanions.
Tetrasodium EDTA is the salt resulting from the neutralization of ethylenediaminetetraacetic acid with four equivalents of sodium hydroxide (or an equivalent sodium base). It is a white solid that is highly soluble in water. Commercial samples are often hydrated, e.g. Na 4 EDTA. 4H 2 O. The properties of solutions produced from the anhydrous ...
TE buffer is a commonly used buffer solution in molecular biology, especially in procedures involving DNA, cDNA or RNA. "TE" is derived from its components: Tris, a common pH buffer, and EDTA, a molecule that chelates cations like Mg 2+. The purpose of TE buffer is to solubilize DNA or RNA, while protecting it from degradation.
It is a white solid that is related to the better known EDTA. Compared to EDTA, it has a lower affinity for magnesium , making it more selective for calcium ions . It is useful in buffer solutions that resemble the environment in living cells [ 3 ] where calcium ions are usually at least a thousandfold less concentrated than magnesium.
TAE buffer is commonly prepared as a 50× stock solution for laboratory use. A 50× stock solution can be prepared by dissolving 242 g Tris base in water, adding 57.1 ml glacial acetic acid, and 100 ml of 500 mM EDTA (pH 8.0) solution, and bringing the final volume up to 1 litre.
Disodium phosphate (DSP), or disodium hydrogen phosphate, or sodium phosphate dibasic, is an inorganic compound with the chemical formula Na 2 H P O 4. It is one of several sodium phosphates. The salt is known in anhydrous form as well as hydrates Na 2 HPO 4 ·nH 2 O, where n is 2, 7, 8, and 12. All are water-soluble white powders. The ...
In ultrafiltration, the molecular weight cut-off or MWCO of a membrane refers to the lowest molecular weight of the solute (in daltons) for which 90% of the solute is retained by (prevented from passing through) the membrane, [1] or the molecular weight of the molecule (e.g. globular protein) that is 90% retained by the membrane.