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Lipofectamine or Lipofectamine 2000 is a common transfection reagent, produced and sold by Invitrogen, used in molecular and cellular biology. [1] It is used to increase the transfection efficiency of RNA (including mRNA and siRNA ) or plasmid DNA into in vitro cell cultures by lipofection . [ 1 ]
Thermo Fisher Scientific's previous headquarters in Waltham, Massachusetts Thermo Fisher Scientific office in Canada. Thermo Fisher Scientific Inc. is an American life, science and clinical research company.
Transfection is the process of deliberately introducing naked or purified nucleic acids into eukaryotic cells. [1] [2] It may also refer to other methods and cell types, although other terms are often preferred: "transformation" is typically used to describe non-viral DNA transfer in bacteria and non-animal eukaryotic cells, including plant cells.
Mediating RNA interference in cultured mammalian cells. Small interfering RNA (siRNA), sometimes known as short interfering RNA or silencing RNA, is a class of double-stranded non-coding RNA molecules, typically 20–24 base pairs in length, similar to microRNA (miRNA), and operating within the RNA interference (RNAi) pathway.
The word liposome derives from two Greek words: lipo ("fat") and soma ("body"); it is so named because its composition is primarily of phospholipid.. Liposomes were first described by British hematologist Alec Douglas Bangham [10] [11] [12] in 1961 at the Babraham Institute, in Cambridge—findings that were published 1964.
The velocity of the charged particle after acceleration will not change since it moves in a field-free time-of-flight tube. The velocity of the particle can be determined in a time-of-flight tube since the length of the path (d) of the flight of the ion is known and the time of the flight of the ion (t) can be measured using a transient digitizer or time to digital converter.
Amiga 3000: 1990–1992 68030 1 MB Chip 1-4 MB Fast 1.3 – 2.04 3.9 / 4.1 FE [note 3] / 3.2 First Zorro III system. Initial machines had a 1.4 beta ROM that looked for a "super" Kickstart disk similar to the 1000. It could load Kickstart versions 1.3, 2.0, and 2.04 this way or from specially named partitions on the hard disk.
3000 1986 61 full MIDI [157] CZ 5000 1985 61 full 8 MIDI 8 track sequencer, juno like sounds, built in chorus (lowers poly), heavy and huge, phase distortion synthesis [158] HT 700 1987 49 mini 8 in/out [159] HT 3000 1987 61 full 8 D (x6) MIDI Programmable synthesizer with additional RAM card slot. Has pitch bender and modulation wheels.