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Lipofectamine or Lipofectamine 2000 is a common transfection reagent, produced and sold by Invitrogen, used in molecular and cellular biology. [1] It is used to increase the transfection efficiency of RNA (including mRNA and siRNA ) or plasmid DNA into in vitro cell cultures by lipofection . [ 1 ]
A difference engine is an automatic mechanical calculator designed to tabulate polynomial functions. It was designed in the 1820s, and was first created by Charles Babbage . The name difference engine is derived from the method of finite differences , a way to interpolate or tabulate functions by using a small set of polynomial co-efficients.
In mathematics, divided differences is an algorithm, historically used for computing tables of logarithms and trigonometric functions. [citation needed] Charles Babbage's difference engine, an early mechanical calculator, was designed to use this algorithm in its operation.
Transfection is the process of deliberately introducing naked or purified nucleic acids into eukaryotic cells. [1] [2] It may also refer to other methods and cell types, although other terms are often preferred: "transformation" is typically used to describe non-viral DNA transfer in bacteria and non-animal eukaryotic cells, including plant cells.
[2] [3] It was first described in 1837 as the successor to Babbage's Difference Engine, which was a design for a simpler mechanical calculator. [ 4 ] The analytical engine incorporated an arithmetic logic unit , control flow in the form of conditional branching and loops , and integrated memory , making it the first design for a general-purpose ...
On a single-step or immediate-execution calculator, the user presses a key for each operation, calculating all the intermediate results, before the final value is shown. [1] [2] [3] On an expression or formula calculator, one types in an expression and then presses a key, such as "=" or "Enter", to evaluate the expression.
The chemical gradient, or difference in solute concentration across a membrane. The electrical gradient, or difference in charge across a membrane. If there are unequal concentrations of an ion across a permeable membrane, the ion will move across the membrane from the area of higher concentration to the area of lower concentration through ...
The TI-83 was the first calculator in the TI series to have built-in assembly language support. The TI-92, TI-85, and TI-82 were capable of running assembly language programs, but only after sending a specially constructed (hacked) memory backup. The support on the TI-83 could be accessed through a hidden feature of the calculator.