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Fluorescence and confocal microscopes operating principle. Confocal microscopy, most frequently confocal laser scanning microscopy (CLSM) or laser scanning confocal microscopy (LSCM), is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out-of-focus light in image formation. [1]
PSF Lab is a software program that allows the calculation of the illumination point spread function (PSF) of a confocal microscope under various imaging conditions. The calculation of the electric field vectors is based on a rigorous, vectorial model that takes polarization effects in the near-focus region and high numerical aperture microscope objectives into account.
The point spread function (PSF) describes the response of a focused optical imaging system to a point source or point object. A more general term for the PSF is the system's impulse response; the PSF is the impulse response or impulse response function (IRF) of a focused optical imaging system. The PSF in many contexts can be thought of as the ...
Amira (ah-MEER-ah) is a software platform for visualization, processing, and analysis of 3D and 4D data. It is being actively developed by Thermo Fisher Scientific in collaboration with the Zuse Institute Berlin (ZIB), and commercially distributed by Thermo Fisher Scientific — together with its sister software Avizo.
Computational imaging systems span a broad range of applications. While applications such as SAR, computed tomography, seismic inversion are well known, they have undergone significant improvements (faster, higher-resolution, lower dose exposures [3]) driven by advances in signal and image processing algorithms (including compressed sensing techniques), and faster computing platforms.
OsiriX is simultaneously a DICOM PACS workstation for imaging and an image processing software package for research (radiology and nuclear imaging), functional imaging, 3D imaging, confocal microscopy and molecular imaging. OsiriX supports a complete plug-in architecture that allows one to expand the capabilities of OsiriX for personal needs.
The optical transfer function of a well-focused (a), and an out-of-focus optical imaging system without aberrations (d). As the optical transfer function of these systems is real and non-negative, the optical transfer function is by definition equal to the modulation transfer function (MTF).
Confocal endoscopy, or confocal laser endomicroscopy (CLE), is a modern imaging technique that allows the examination of real-time microscopic and histological features inside the body. In the word "endomicroscopy", endo- means "within" and -skopein means "to view or observe".
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