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If you want to master the art of interpreting a chromatogram, you first need to know exactly what a chromatogram is. But before moving on to that, let’s first take a look at chromatography, its advantages, types, and other details that will further help in the understanding of a chromatogram.
In chemical analysis, chromatography is a laboratory technique for the separation of a mixture into its components. The mixture is dissolved in a fluid solvent (gas or liquid) called the mobile phase, which carries it through a system (a column, a capillary tube, a plate, or a sheet) on which a material called the stationary phase is fixed.
The time to elute an analyte is a function of how long the analyte is retained on the column, therefore the output of IC is a graph of conductivity as a function of time, called a chromatogram. Based on the previous discussion of elution, you may expect a chromatogram to look something like:
The chromatogram and the related data from the detector help us calculate the concentration of each compound. The detector basically responds to the concentration of the compound band as it passes through the flow cell.
Chromatography, technique for separating the components, or solutes, of a mixture on the basis of the relative amounts of each solute distributed between a moving fluid stream, called the mobile phase, and a contiguous stationary phase. Learn more about chromatography in this article.
The only thing you will need to know about how chromatography works to follow this video, is that they all separate compounds based on how they interact with...
Chromatography is a method by which a mixture is separated by distributing its components between two phases. The stationary phase remains fixed in place while the mobile phase carries the components ….