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  2. Cell culture - Wikipedia

    en.wikipedia.org/wiki/Cell_culture

    Cell culture is a fundamental component of tissue culture and tissue engineering, as it establishes the basics of growing and maintaining cells in vitro. The major application of human cell culture is in stem cell industry, where mesenchymal stem cells can be cultured and cryopreserved for future use.

  3. Tissue culture - Wikipedia

    en.wikipedia.org/wiki/Tissue_culture

    Tissue culture is an important tool for the study of the biology of cells from multicellular organisms. It provides an in vitro model of the tissue in a well defined environment which can be easily manipulated and analysed. In animal tissue culture, cells may be grown as two-dimensional monolayers (conventional culture) or within fibrous ...

  4. Plant tissue culture - Wikipedia

    en.wikipedia.org/wiki/Plant_tissue_culture

    Plant tissue culture is a collection of techniques used to maintain or grow plant cells, tissues, or organs under sterile conditions on a nutrient culture medium of known composition. It is widely used to produce clones of a plant in a method known as micropropagation. Different techniques in plant tissue culture may offer certain advantages ...

  5. Micropropagation - Wikipedia

    en.wikipedia.org/wiki/Micropropagation

    Micropropagation or tissue culture is the practice of rapidly multiplying plant stock material to produce many progeny plants, using modern plant tissue culture methods. [1] Micropropagation is used to multiply a wide variety of plants, such as those that have been genetically modified or bred through conventional plant breeding methods.

  6. Primary cell culture - Wikipedia

    en.wikipedia.org/wiki/Primary_cell_culture

    Primary cell culture is the ex vivo culture of cells freshly obtained from a multicellular organism, as opposed to the culture of immortalized cell lines.In general, primary cell cultures are considered more representative of in vivo tissues than cell lines, and this is recognized legally in some countries such as the UK (Human Tissue Act 2004). [1]

  7. Trypsinization - Wikipedia

    en.wikipedia.org/wiki/Trypsinization

    Trypsinization is the process of cell dissociation using trypsin, a proteolytic enzyme which breaks down proteins, to dissociate adherent cells from the vessel in which they are being cultured. When added to cell culture, trypsin breaks down the proteins that enable the cells to adhere to the vessel. Trypsinization is often used to pass cells ...

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