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In an aqueous solution, precipitation is the "sedimentation of a solid material (a precipitate) from a liquid solution". [1] [2] The solid formed is called the precipitate. [3] In case of an inorganic chemical reaction leading to precipitation, the chemical reagent causing the solid to form is called the precipitant. [4]
Protein precipitation is widely used in downstream processing of biological products in order to concentrate proteins and purify them from various contaminants. For example, in the biotechnology industry protein precipitation is used to eliminate contaminants commonly contained in blood. [1]
Salting out is typically used to precipitate large biomolecules, such as proteins or DNA. [2] Because the salt concentration needed for a given protein to precipitate out of the solution differs from protein to protein, a specific salt concentration can be used to precipitate a target protein. This process is also used to concentrate dilute ...
If the substance lacks the ability to dissolve in water, the molecules form a precipitate. [3] When writing the equations of precipitation reactions, it is essential to determine the precipitate. To determine the precipitate, one must consult a chart of solubility. Soluble compounds are aqueous, while insoluble compounds are the precipitate.
In chemistry, coprecipitation (CPT) or co-precipitation is the carrying down by a precipitate of substances normally soluble under the conditions employed. [1] Analogously, in medicine, coprecipitation (referred to as immunoprecipitation) is specifically "an assay designed to purify a single antigen from a complex mixture using a specific antibody attached to a beaded support".
Immunoprecipitation of intact protein complexes (i.e. antigen along with any proteins or ligands that are bound to it) is known as co-immunoprecipitation (Co-IP). Co-IP works by selecting an antibody that targets a known protein that is believed to be a member of a larger complex of proteins.
Gravimetric analysis describes a set of methods used in analytical chemistry for the quantitative determination of an analyte (the ion being analyzed) based on its mass. The principle of this type of analysis is that once an ion's mass has been determined as a unique compound, that known measurement can then be used to determine the same analyte's mass in a mixture, as long as the relative ...
100 mg of sample tannins are dissolved in 10 ml distilled water. 1 ml of 10M HCl and 2 ml of 37% formaldehyde are added and the mixture heated under reflux for 30 min. The reaction mixture is filtered while hot through a sintered glass filter. The precipitate is washed with hot water (5× 10 ml) and dried over CaCl 2. The yield of tannin is ...