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In science, ex vivo refers to experimentation or measurements done in or on tissue from an organism in an external environment with minimal alteration of natural conditions. [ 2 ] A primary advantage of using ex vivo tissues is the ability to perform tests or measurements that would otherwise not be possible or ethical in living subjects.
Ex vivo reconstruction, short for ex vivo renal artery reconstruction and autotransplantation, is a technique mainly used for complex disease involving multiple segmental branches in patients with fibromuscular dysplasia.
Autotransplantation of selected organs is often preceded by ex vivo (also bench, back-table, or extracorporeal) surgery. [6] For example, ex vivo liver resection and autotransplantation is used in the treatment of selected cases of conventionally unresectable hepatic tumors. [7] It can also be implemented in rare scenarios of a blunt abdominal ...
Primary cell culture is the ex vivo culture of cells freshly obtained from a multicellular organism, as opposed to the culture of immortalized cell lines.In general, primary cell cultures are considered more representative of in vivo tissues than cell lines, and this is recognized legally in some countries such as the UK (Human Tissue Act 2004). [1]
Ex vivo lung perfusion, EVLP, is a form of machine perfusion aimed at sustaining the active aerobic cellular metabolism of donor lungs outside the donor's body prior to lung transplantation. This medical preservation technique typically occurs within a specialised machine engineered to mimic the conditions of the natural circulatory system .
These techniques can be performed either in vivo, ex vivo, or in vitro. [3] Ex vivo techniques enable a more accurate count of the T cells in a graft and also has the option to 'addback' a set number of T cells if necessary. Currently, ex vivo techniques most commonly employ positive or negative selection methods using immunomagnetic separation.
The Langendorff heart or isolated perfused heart assay is an ex vivo technique used in pharmacological and physiological research using animals and also humans. [1] Named after the German physiologist Oskar Langendorff , this technique allows the examination of cardiac contractile strength and heart rate without the complications of an intact ...
Hypothermic perfusion of kidneys is a relatively widespread practice. For the heart normothermic preservation has been used in which the heart is provided with warm oxygenated blood and so continues to beat ex-vivo during its preservation.