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Universal primer hybridises to the RC probe and is extended by the DNA polymerase generating target specific primer. The 5 prime portion of the RC probe contains the reverse complement sequence of the desired target specific primer sequence. In RC-PCR, no target specific primers are present in the reaction mixture.
After sequencing the PCR products, the generated reads divide into tag families based on the genomic position, duplex tags, and the neighboring sequencing adapter. Sequence tag α is the reverse complement of sequence tag β and vice versa.
An inverted repeat (or IR) is a single stranded sequence of nucleotides followed downstream by its reverse complement. [1] The intervening sequence of nucleotides between the initial sequence and the reverse complement can be any length including zero. For example, 5'---TTACGnnnnnn CGTAA---3' is an inverted repeat sequence.
Partial sequences of cDNAs are often obtained as expressed sequence tags. With amplification of DNA sequences via polymerase chain reaction (PCR) now commonplace, one will typically conduct reverse transcription as an initial step, followed by PCR to obtain an exact sequence of cDNA for intra-cellular expression. This is achieved by designing ...
Gene conversion is the process by which one DNA sequence replaces a homologous sequence such that the sequences become identical after the conversion. [1] Gene conversion can be either allelic, meaning that one allele of the same gene replaces another allele, or ectopic, meaning that one paralogous DNA sequence converts another.
In nature complementarity is the base principle of DNA replication and transcription as it is a property shared between two DNA or RNA sequences, such that when they are aligned antiparallel to each other, the nucleotide bases at each position in the sequences will be complementary, much like looking in the mirror and seeing the reverse of things.
For example, the built-in reverse complement utility reverses the order of characters and replaces each with its complement. [17] The screenshots demonstrate the use of MEGA's reverse complement tool. The original sequence was reversed and each nucleotide was replaced with its complement to produce the reverse complement.
DNA polymerase moves along a strand of DNA, creating its complementary strand. The original strand is washed away, leaving only the reverse strand. At the top of the reverse strand there is an adapter sequence. The DNA strand bends and attaches to the oligo that is complementary to the top adapter sequence.