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In the fields of histology, pathology, and cell biology, fixation is the preservation of biological tissues from decay due to autolysis or putrefaction.It terminates any ongoing biochemical reactions and may also increase the treated tissues' mechanical strength or stability.
Cryofixation is a technique for fixation or stabilisation of biological materials as the first step in specimen preparation for the electron microscopy and cryo-electron microscopy. [1] Typical specimens for cryofixation include small samples of plant or animal tissue , cell suspensions of microorganisms or cultured cells , suspensions of ...
Plant microtechnique involves classical methods developed over a hundred years ago and new methods developed to expand our research scope and depth in botanical micro studies. [15] Both traditional and new micro technique is useful for experimental research, and some will have a significant influence on further study. [ 3 ]
Zenker's fixative is a rapid-acting fixative for animal tissues. It is employed to prepare specimens of animal or vegetable tissues for microscopic study. It provides excellent fixation of nuclear chromatin, connective tissue fibers and some cytoplasmic features, but does not preserve delicate cytoplasmic organelles such as mitochondria.
Immunohistochemistry can be performed on tissue that has been fixed and embedded in paraffin, but also cryopreservated (frozen) tissue.Based on the way the tissue is preserved, there are different steps to prepare the tissue for immunohistochemistry, but the general method includes proper fixation, antigen retrieval incubation with primary antibody, then incubation with secondary antibody.
Histologic specimen being placed on the stage of an optical microscope Human lung tissue stained with hematoxylin and eosin as seen under a microscope. Histology, [help 1] also known as microscopic anatomy or microanatomy, [1] is the branch of biology that studies the microscopic anatomy of biological tissues.
This category contains scientific and medical journals covering microbiology and infectious diseases. Subcategories This category has the following 6 subcategories, out of 6 total.
Histological characterisation [ edit ] Silver staining aids the visualization of targets of interest, namely intracellular and extracellular cellular components such as DNA and proteins , such as type III collagen and reticulin fibres by the deposition of metallic silver particles on the targets of interest.