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Iteron plasmids control copy number through two combined methods, suitable for low copy number stringent plasmids. One method is control of RepA synthesis. RepA is the only plasmid-encoded protein required for replication in pSC101.
The plasmid pSC101, a natural plasmid from Salmonella panama, [5] confers tetracycline resistance which allows for simpler screening process with antibiotic selection, but it is a low copy number plasmid which does not give a high yield of plasmid. Another plasmid, RSF 2124, which is a derivative of ColE1, confers ampicillin resistance but is ...
Larger plasmids tend to have lower copy numbers. [15] Low-copy-number plasmids that exist only as one or a few copies in each bacterium are, upon cell division, in danger of being lost in one of the segregating bacteria. Such single-copy plasmids have systems that attempt to actively distribute a copy to both daughter cells.
The R1 plasmid imparts multi-drug antibiotic resistance to its host bacteria. [3] It's known as a "low copy" plasmid, meaning that it exists in relatively few copies in any given bacteria. This characteristic allows the R1 plasmid to have an efficient plasmid stabilization system, that aids in stabilizing medium copy number plasmids.
Low Copy Number (LCN) is a DNA profiling technique developed by the UK Forensic Science Service (FSS) which has been in use since 1999. [1]In the United Kingdom use of the technique was suspended between 21 December 2007 and 14 January 2008 while the Crown Prosecution Service conducted a review into its use – this suspension has now been lifted.
The relatively large size of the plasmid requires [1] it to keep a low copy number lest it become too large a metabolic burden while it is a lysogen. As there is usually only one copy of the plasmid per bacterial genome, the plasmid stands a high chance of not being passed to both daughter cells. [5] The P1 plasmid combats this by several methods:
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