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The depth of field (DOF) is the distance between the nearest and the farthest objects that are in acceptably sharp focus in an image captured with a camera.
The principles of LSP were first documented in the early 1960s by Dan McLachlan Jr., who highlighted its capability for extreme focal depth in microscopy [1] and in 1968 patented the process. [ 2 ] The technique was revived and further developed in the 1980s by photographers such as Darwin Dale and Nile Root, a faculty member at the Rochester ...
Focus stacking (for extended depth of field) in bright field light microscopy. This example is of a diatom microfossil in diatomaceous earth. Three source images at different focus distances (top left) are combined with masks (top right) to obtain the contributions of their respective images to the final focus stacked image (bottom).
Antonie van Leeuwenhoek (1632–1723). The field of microscopy (optical microscopy) dates back to at least the 17th-century.Earlier microscopes, single lens magnifying glasses with limited magnification, date at least as far back as the wide spread use of lenses in eyeglasses in the 13th century [2] but more advanced compound microscopes first appeared in Europe around 1620 [3] [4] The ...
With no modification to the microscope, i.e. with a simple wide field light microscope, the quality of optical sectioning is governed by the same physics as the depth of field effect in photography. For a high numerical aperture lens, equivalent to a wide aperture, the depth of field is small (shallow focus) and gives
Diagram illustrating near-field optics, with the diffraction of light coming from NSOM fiber probe, showing wavelength of light and the near-field. [1] Comparison of photoluminescence maps recorded from a molybdenum disulfide flake using NSOM with a campanile probe (top) and conventional confocal microscopy (bottom).
More important, though, is that microscope-based systems have less depth of field issues generally versus dynamic imaging systems. This is because the sample is placed on a microscope slide, and then usually covered with a cover slip, thus limiting the plane containing the particles relative to the optical axis. This means that more particles ...
Great working distance and depth of field are important qualities for this type of microscope. Both qualities are inversely correlated with resolution: the higher the resolution (i.e. the greater the distance at which two adjacent points can be distinguished as separate), the smaller the depth of field and working distance. Some stereo ...