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Gram stain (Gram staining or Gram's method), is a method of staining used to classify bacterial species into two large groups: gram-positive bacteria and gram-negative bacteria. It may also be used to diagnose a fungal infection. [1] The name comes from the Danish bacteriologist Hans Christian Gram, who developed the technique in 1884. [2]
The pink blobs are vaginal epithelial cells and the dark granules are common artefacts of the Gram-stain. (Note: microscope lenses do not have F-numbers they have Numerical apertures, this one was NA 1.25 and oil immersion). Articles in which this image appears Candidiasis, Candida albicans, Yeast, Gram stain, Chlamydospore, Fungus
Both gram-positive and gram-negative bacteria commonly have a surface layer called an S-layer. In gram-positive bacteria, the S-layer is attached to the peptidoglycan layer. Gram-negative bacteria's S-layer is attached directly to the outer membrane. Specific to gram-positive bacteria is the presence of teichoic acids in the cell wall. Some of ...
In Berlin, in 1884, Gram developed a method for distinguishing between two major classes of bacteria. [1] This technique, known as Gram staining, continues to be a standard procedure of medical microbiology. This work gained Gram an international reputation. The staining method later played a major role in classifying bacteria. Gram was a ...
Veillonella are Gram-negative bacteria (Gram stain pink) anaerobic cocci, unlike most Bacillota, which are Gram-positive bacteria. [1] This bacterium is well known for its lactate fermenting abilities. It is a normal bacterium in the intestines and oral mucosa of mammals.
Clostridium tetani is a rod-shaped, Gram-positive bacterium, typically up to 0.5 μm wide and 2.5 μm long. [1] It is motile by way of various flagella that surround its body. [1] C. tetani cannot grow in the presence of oxygen. [1] It grows best at temperatures ranging from 33 to 37 °C. [1]
Micrograph of a clue cell (center), covered in bacteria, as compared to an unremarkable squamous cell at bottom left, and a neutrophil at bottom center. Pap stain Phase contrast microscopy of clue cells in a vaginal swab. Clue cells are epithelial cells of the vagina that get their distinctive stippled appearance by being covered with bacteria ...
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