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Klebsiella aerogenes, [2] previously known as Enterobacter aerogenes, is a Gram-negative, oxidase-negative, catalase-positive, citrate-positive, indole-negative, rod-shaped bacterium. [3] Capable of motility via peritrichous flagella, [ 4 ] it is approximately one to three microns in length.
On carbohydrate-rich media, Klebsiella colonies appear greyish-white in colour with a mucosal outer surface. [6] The media used for selecting for Klebsiella species in a mixed sample is an agar including ornithine, raffinose, and Koser citrate, where members of this genus will form yellow, wet-looking colonies. [8]
Klebsiella pneumoniae is a Gram-negative, non-motile, encapsulated, lactose-fermenting, facultative anaerobic, rod-shaped bacterium. It appears as a mucoid lactose fermenter on MacConkey agar .
E. coli on EMB agar. Eosin methylene blue (EMB, also known as "Levine's formulation") is a selective and differential media used for the identification of Gram-negative bacteria, [1] specifically the Enterobacteriaceae. EMB inhibits the growth of most Gram-positive bacteria. EMB is often used to confirm the presence of coliforms in a sample.
Klebsiella is a genus of Gram-negative, oxidase-negative, rod-shaped bacteria with a prominent polysaccharide-based capsule. [ 3 ] Klebsiella is named after German-Swiss microbiologist Edwin Klebs (1834–1913).
Buffered charcoal yeast extract (BCYE) agar is a selective growth medium used to culture or grow certain types of bacteria, particularly the Gram-negative species Legionella pneumophila. [1] It has also been used for the laboratory diagnosis of Acanthamoeba keratitis , [ 2 ] Francisella and Nocardia spp .
Klebsiella oxytoca on agar plate. Klebsiella oxytoca is a Gram-negative, rod-shaped bacterium that is closely related to K. pneumoniae, from which it is distinguished by being indole-positive; it also has slightly different growth characteristics in that it is able to grow on melezitose, but not 3-hydroxybutyrate.
Mueller Hinton agar is commonly used in the disk diffusion method, which is a simple and widely used method for testing the susceptibility of bacterial isolates to antibiotics. In this method, small disks impregnated with different antibiotics are placed on the surface of the agar, and the zone of inhibition around each disk is measured to ...