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  2. Plant tissue culture - Wikipedia

    en.wikipedia.org/wiki/Plant_tissue_culture

    Plant tissue culture is used widely in the plant sciences, forestry, and horticulture. Applications include: The commercial production of plants used as potting, landscape, and florist subjects, which uses meristem and shoot culture to produce large numbers of identical individuals. [8] To conserve rare or endangered plant species. [9] [10]

  3. Micropropagation - Wikipedia

    en.wikipedia.org/wiki/Micropropagation

    Micropropagation or tissue culture is the practice of rapidly multiplying plant stock material to produce many progeny plants, using modern plant tissue culture methods. [ 1 ] Micropropagation is used to multiply a wide variety of plants, such as those that have been genetically modified or bred through conventional plant breeding methods.

  4. Tissue culture - Wikipedia

    en.wikipedia.org/wiki/Tissue_culture

    Plant tissue culture in particular is concerned with the growing of entire plants from small pieces of plant tissue, cultured in medium. [10] The technique of plant tissue culture, i.e., culturing plant cells or tissues in artificial medium supplemented with required nutrients, has many applications in efficient clonal propagation (true to the ...

  5. Cell culture - Wikipedia

    en.wikipedia.org/wiki/Cell_culture

    Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants. The lifespan of most cells is genetically determined, but some cell-culturing cells have been 'transformed' into immortal cells which will reproduce indefinitely if the optimal conditions are provided.

  6. Explant culture - Wikipedia

    en.wikipedia.org/wiki/Explant_culture

    In biology, explant culture is a technique to organotypically culture cells from a piece or pieces of tissue or organ removed from a plant or animal. The term explant can be applied to samples obtained from any part of the organism. The extraction process is extensively sterilized, and the culture can be typically used for two to three weeks. [1]

  7. Vegetative reproduction - Wikipedia

    en.wikipedia.org/wiki/Vegetative_reproduction

    In tissue culture, plant cells are taken from various parts of the plant and are cultured and nurtured in a sterilized medium. The mass of developed tissue, known as the callus, is then cultured in a hormone-ladened medium and eventually develops into plantlets which are then planted and eventually develop into grown plants. [12] [32]

  8. Subculture (biology) - Wikipedia

    en.wikipedia.org/wiki/Subculture_(biology)

    In biology, a subculture is either a new cell culture or a microbiological culture made by transferring some or all cells from a previous culture to fresh growth medium. This action is called subculturing or passaging the cells. Subculturing is used to prolong the lifespan and/or increase the number of cells or microorganisms in the culture. [1]

  9. Microtechnique - Wikipedia

    en.wikipedia.org/wiki/Microtechnique

    The substance used to embed tissue is embedding media, which is chosen depends on the category of the microscope, category of the micro tome, and category of tissue. [23] Paraffin wax, whose melting point is from 56 to 62°C, is commonly used for embedding. [22] Tissue processing - Tissue sections on slides are stained on an automated stainer