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To perform the OF-glucose test, two tubes of OF-glucose medium are inoculated with the test organism. A layer of mineral oil is added to the top of the deep in one of the tubes to create anaerobic conditions. Oil is not added to the other tube to allow for aerobic conditions. The tubes are then incubated for 24–48 hours.
The flame test is a more well known version of the chemical test. It is only used on metallic ions. The metal powder is burned, causing an emission of colors based on what metal was burned. For example, calcium (Ca) will burn orange and copper (Cu) will burn blue. Their color emissions are used to produce bright colors in fireworks. [citation ...
Colorimetric analysis is a method of determining the concentration of a chemical element or chemical compound in a solution with the aid of a color reagent.It is applicable to both organic compounds and inorganic compounds and may be used with or without an enzymatic stage.
Substance in water + 3 mL Benedict's solution, then boil for few minutes and allow to cool. Red, green, or yellow precipitate is obtained Reducing sugar, such as glucose, is present Substance in water + 3 mL Benedict's solution, then boil for few minutes and allow to cool. Solution remains clear or is a little blue Reducing sugar is not present
The aqueous solution in the classical reaction contains glucose, sodium hydroxide and methylene blue. [14] In the first step an acyloin of glucose is formed. The next step is a redox reaction of the acyloin with methylene blue in which the glucose is oxidized to diketone in alkaline solution [6] and methylene blue is reduced to colorless leucomethylene blue.
The volumetric titration is based on the same principles as the coulometric titration, except that the anode solution above now is used as the titrant solution. The titrant consists of an alcohol (ROH), base (B), SO 2 and a known concentration of I 2. Pyridine has been used as the base in this case. One mole of I 2 is consumed for each mole of ...
The test employs the Trinder reagent, and is a colour change test resulting from the Trinder reaction. The Trinder reagent, named after P. Trinder of the Biochemistry Department of the Royal Infirmary in Sunderland (see the article listed in further reading ), comprises an aminoantipyrine (such as 4-aminoantipyrine ) and phenol (p-hydroxybenzene).
Stabilisation : depending on the nature of the coating ingredient(s) and substrate product, the ingredient is stabilised by elimination of the solvent (drying and evaporation of water, alcohol), crystallisation (sugar crystallises when water is evaporated, fat crystallises when cooled), or thermal treatment (proteins set irreversibly when heated).