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The Van Slyke determination is a chemical test for the determination of amino acids containing a primary amine group. It is named after the biochemist Donald Dexter Van Slyke (1883-1971). [1] One of Van Slyke's first professional achievements was the quantification of amino acids by the Van Slyke determination reaction. [2]
Donald Dexter Van Slyke (March 29, 1883 – May 4, 1971), nicknamed Van, was a Dutch American biochemist. His achievements included the publication of 317 journal articles and 5 books, [ 1 ] as well as numerous awards, among them the National Medal of Science and the first AMA Scientific Achievement Award . [ 1 ]
The model is used in a variety of biochemical situations other than enzyme-substrate interaction, including antigen–antibody binding, DNA–DNA hybridization, and protein–protein interaction. [ 17 ] [ 18 ] It can be used to characterize a generic biochemical reaction, in the same way that the Langmuir equation can be used to model generic ...
With nitrous acid, one obtains glycolic acid (van Slyke determination). With methyl iodide, the amine becomes quaternized to give trimethylglycine, a natural product: H 3 N + CH 2 COO − + 3 CH 3 I → (CH 3) 3 N + CH 2 COO − + 3 HI. Glycine condenses with itself to give peptides, beginning with the formation of glycylglycine: [34] 2 H 3 N ...
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By contrast, at almost the same time, Donald Van Slyke and G. E. Cullen [79] treated the binding step as an irreversible reaction. The Briggs–Haldane equation was of the same algebraic form as both of the earlier equations, but their derivation is based on the quasi- steady state approximation, which is the concentration of intermediate ...
The method combines the reactions of copper ions with the peptide bonds under alkaline conditions (the Biuret test) with the oxidation of aromatic protein residues. The Lowry method is based on the reaction of Cu +, produced by the oxidation of peptide bonds, with Folin–Ciocalteu reagent (a mixture of phosphotungstic acid and phosphomolybdic acid in the Folin–Ciocalteu reaction).
Fehling's solution is prepared by combining two separate solutions: Fehling's A, which is a deep blue aqueous solution of copper(II) sulfate, and Fehling's B, which is a colorless solution of aqueous potassium sodium tartrate (also known as Rochelle salt) made strongly alkaline with sodium hydroxide.