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Scheme of solid-phase peptides synthesis (SPPS) on a resin as solid support with protected amino acids. The deprotection is usually done using a base such as piperidine. This is followed by a coupling step (a protected amino acid is added) to the growing peptide chain. Coupling reagents (e.g. HBTU, HATU, or DIC) are employed to help form the ...
DEPBT (3-(diethoxyphosphoryloxy)-1,2,3-benzotriazin-4(3H)-one) is a peptide coupling reagent used in peptide synthesis.It shows remarkable resistance to racemization. [1]Fmoc-Dab(Mtt)-OH, a commercially available amino acid building block for solid-phase peptide synthesis (SPPS), was proven to undergo rapid lactamization, instead of reacting with the N-terminal end of the peptide.
HBTU (Hexafluorophosphate Benzotriazole Tetramethyl Uronium) is a coupling reagent used in solid phase peptide synthesis. It was introduced in 1978 and shows resistance against racemization. [3] [4] It is used because of its mild activating properties. [5]
The primary advantage the Edman degradation has over the Bergmann degradation is the ease with which the residual peptide can re-enter the process due to retention of its structure throughout sequential cleaving. [5] [6] Repetition of the Bergmann degradation is presumably not as straightforward, as the remaining peptide is in amide form. [3]
The most common way of targeting a protein of interest is by fusing the protein at its N-terminus, C-terminus or at an internal loop to a 15 amino acid peptide (GLNDIFEAQKIEWHE), termed AviTag or Acceptor Peptide (AP). [5] Once tagged, the protein is then incubated with BirA allowing biotinylation to take place in the presence of biotin and ATP ...
N,N′-Dicyclohexylcarbodiimide (DCC or DCCD) [1] is an organic compound with the chemical formula (C 6 H 11 N) 2 C. It is a waxy white solid with a sweet odor. Its primary use is to couple amino acids during artificial peptide synthesis.
The payoff in the native chemical ligation method is that coupling long peptides by this technique is typically near quantitative and provides synthetic access to large peptides and proteins otherwise impossible to make, due to their large size, decoration by post-translational modification, and containing non-coded amino acid or other chemical ...
Peptide synthesis from NCAs does not require protection of the amino acid functional groups. N-Substituted NCAs, such as sulfenamide derivatives have also been examined. [15] The ring-opening polymerization of NCAs is catalyzed by metal catalysts. [16] [3] [6] [10] The polymerization of NCA’s have been considered as a prebiotic route to ...