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When the acoustic wave propagates though the sample it may be scattered, absorbed or reflected at media interfaces. Thus, the technique registers the echo generated by the acoustic impedance (Z) contrast between two materials. Scanning acoustic microscopy works by directing focused sound from a transducer at a small point on a target object.
Advancements of this instrument, a scanning acoustic microscope, have to do with achieving very high resolution, novel modes of imaging, and applications. The SAM was commercially introduced by Leitz Corp and by Olympus Corp. In 1970, the Korpel and Kessler group began to pursue a scanning laser detection system for acoustic microscopy. [8]
Confocal microscopy: Fluorescence, scattering 0.2 3-20 0.3-3 10-100 Two-photon microscopy: Fluorescence 0.5-1.0 1-10 0.3-3 10-100 Optical coherence tomography: 300 T Optical scattering 1-2 0.5-10 1-10 20-4.000 Scanning laser acoustic microscopy: 300 M Ultrasonic scattering 1-2 20 20 10 Acoustic microscopy: 50 M Ultrasonic scattering 20 20-100 ...
Fluorescence and confocal microscopes operating principle. Confocal microscopy, most frequently confocal laser scanning microscopy (CLSM) or laser scanning confocal microscopy (LSCM), is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out-of-focus light in image formation. [1]
AFAM. Atomic force acoustic microscopy (AFAM) is a type of scanning probe microscopy (SPM). It is a combination of acoustics and atomic force microscopy. The principal difference between AFAM and other forms of SPM is the addition of a transducer at the bottom of the sample which induces longitudinal out-of-plane vibrations in the specimen.
Photoacoustic microscopy takes advantage of the local temperature rise that occurs as a result of light absorption in tissue. Using a nanosecond pulsed laser beam, tissues undergo thermoelastic expansion, resulting in the release of a wide-band acoustic wave that can be detected using a high-frequency ultrasound transducer. [1]
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