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Nonidet P-40 is a nonionic, non-denaturing detergent. Its official IUPAC name is octylphenoxypolyethoxyethanol. Nonidet P-40 is sometimes abbreviated as NP-40, but should not be confused with a different detergent by the same name NP-40 , nonylphenoxypolyethoxyethanol of the Tergitol NP series of Dow Chemicals.
IGEPAL CA-630 has HLB of 13.4, similar to that of Triton X-100 (13.4) and thus belongs to the detergent range (HLB 13-15); this is significantly less than 17.8 of tergitol NP-40 or 16.7 of Polysorbate 20 (also known as Tween 20), which both belong in the solubilizer range (15-18) of HLB. [2] Ca-630 is completely miscible with water.
Confusingly, biochemical protocols published between the 1960s [3] and 2000s refer to Shell's Nonidet P-40 as NP-40. Shell's original Nonidet P-40 had a hydrophilic-lipophilic balance (HLB) value of 13.5, [ 4 ] as opposed to 12.9 for the currently available IGEPAL CA-630 , [ 5 ] indicating that the currently available compound is more potent ...
Triton X-100 is closely related to IGEPAL CA-630, which might differ from it mainly in having slightly shorter ethylene oxide chains. As a result, Triton X-100 is slightly more hydrophilic than Igepal CA-630 thus these two detergents may not be considered functionally interchangeable for most applications. [3]
Radioimmunoprecipitation assay buffer (RIPA buffer) is a lysis buffer used to lyse cells and tissue for the radio immunoprecipitation assay (RIPA). [1] [2] This buffer is more denaturing than NP-40 or Triton X-100 because it contains the ionic detergents SDS and sodium deoxycholate as active constituents and is particularly useful for disruption of nuclear membranes in the preparation of ...
The solubilizing agent is NP-40, which can be replaced by other detergents at different concentrations. Since NP-40 is a nonionic detergent, this lysis buffer has a milder effect than RIPA buffer. It can be used when protein functions are to be retained with minimal disruption. [9] Recipe: [9] 150 mM NaCl; 1.0% Nonidet P-40 or Triton X-100; 50 ...
The Environmental quality standard for NP was proposed to be 0.3 ug/L. [2] In 2013 nonylphenols were registered on the REACH candidate list. In the US, the EPA set criteria which recommends that nonylphenol concentration should not exceed 6.6 ug/L in fresh water and 1.7 ug/L in saltwater. [ 52 ]
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