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The recipes emerged from Claude L. Pierre Masson's (1880–1959) original formulation have different specific applications, but all are suited for distinguishing cells from surrounding connective tissue. Most recipes produce red keratin and muscle fibers, blue or green collagen and bone, light red or pink cytoplasm, and dark brown to black cell ...
Sirius Red F 3B (Direct Red 80) is an azo dye primarily used in staining methods for collagen and amyloid. [1] It has the molecular formula C 45 H 26 N 10 Na 6 O 21 S 6.. In histology, sirius red staining is used in various domains of diagnostic to observe fibrosis levels in a lot of cases of inflammation induced by cancer, vascular or metabolic pathologies.
The first staining protocol that was described as "trichrome" was Mallory's trichrome stain, which differentially stained erythrocytes to a red colour, muscle tissue to a red colour, and collagen to a blue colour. Some other trichrome staining protocols are the Masson's trichrome stain, Lillie's trichrome, and the Gömöri trichrome stain.
Spindle cell lipoma Angiosarcoma Dermatofibrosarcoma protuberans Atypical fibroxanthoma Pleomorphic fibroma Intravascular large B-cell lymphoma: CD38: Plasma cell: Multiple myeloma Plasmablastic lymphoma Pagetoid reticulosis: CD44: Merkel cell carcinoma: CD56: Extranodal natural killer cell lymphoma Merkel cell carcinoma: CD68: Benign cephalic ...
Masson's trichrome staining on rat trachea. Hematoxylin and eosin is one of the most commonly used stains in histology to show the general structure of the tissue. [9] [19] Hematoxylin stains cell nuclei blue; eosin, an acidic dye, stains the cytoplasm and other tissues in different stains of pink. [9] [12]
Rouleaux formation on wet smear. Rouleaux (singular is rouleau) are stacks or aggregations of red blood cells (RBCs) that form because of the unique discoid shape of the cells in vertebrates. The flat surface of the discoid RBCs gives them a large surface area to make contact with and stick to each other; thus forming a rouleau.
Main staining types when using hematoxylin and eosin (H&E). A Basophil granulocyte stains dark purple upon H&E staining. Basophilic is a technical term used by pathologists. It describes the appearance of cells, tissues and cellular structures as seen through the microscope after a histological section has been stained with a basic dye.
It is similar to Masson's trichrome stain, but it uses Biebrich scarlet for the plasma stain. It was initially published by Ralph D. Lillie in 1940. [ 1 ] It is applied by submerging the fixated sample into the following three solutions: [ 2 ] Weigert's iron hematoxylin working solution, Biebrich scarlet solution, and Fast Green FCF solution.