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Illustration of streak plate procedure to achieve isolated colonies using aseptic technique. The three-phase streaking pattern, known as the T-Streak, is recommended for beginners. The streaking is done using a sterile tool, such as a cotton swab or commonly an inoculation loop. The inoculation loop is first sterilized by passing it through a ...
The phage can then be isolated from the resulting plaques in a lawn of bacteria on a plate. Viral cultures are obtained from their appropriate eukaryotic host cells. The streak plate method is a way to physically separate the microbial population, and is done by spreading the inoculate back and forth with an inoculating loop over the solid agar ...
A third technique is using sterile glass beads to plate out cells. In this technique, cells are grown in a liquid culture, in which a small volume is pipetted on the agar plate and then spread out with the beads. Replica plating is another technique used to plate out cells on agar plates. These four techniques are the most common, but others ...
Where the plates meet, their relative motion determines the type of plate boundary (or fault): convergent, divergent, or transform. The relative movement of the plates typically ranges from zero to 10 cm annually. [5] Faults tend to be geologically active, experiencing earthquakes, volcanic activity, mountain-building, and oceanic trench formation.
An agar plate – an example of a bacterial growth medium*: Specifically, it is a streak plate; the orange lines and dots are formed by bacterial colonies.. A growth medium or culture medium is a solid, liquid, or semi-solid designed to support the growth of a population of microorganisms or cells via the process of cell proliferation [1] or small plants like the moss Physcomitrella patens. [2]
Convergent boundaries are areas where plates move toward each other and collide. These are also known as compressional or destructive boundaries. Obduction zones occurs when the continental plate is pushed under the oceanic plate, but this is unusual as the relative densities of the tectonic plates favours subduction of the oceanic plate. This ...
The relative movement of the plates typically ranges from zero to 10 cm annually. Faults tend to be geologically active, experiencing earthquakes, volcanic activity, mountain-building, and oceanic trench formation. Tectonic plates are composed of the oceanic lithosphere and the thicker continental lithosphere, each topped by its own kind of crust.
Beginning the streak pattern. Label the base of the plate. Then, visualize the plate in four quadrants: top left (I), top right (II), bottom right (III), bottom left (IV). Streak the mixed culture back and forth in the first quadrant (top left) of the agar plate. Do not cut the agar, simply scrape the top. Flame the loop to rid of culture residue.