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Endomicroscopy is a technique for obtaining histology-like images from inside the human body in real-time, [1] [2] [3] a process known as ‘optical biopsy’. [4] [5] It generally refers to fluorescence confocal microscopy, although multi-photon microscopy and optical coherence tomography have also been adapted for endoscopic use.
Confocal endoscopy, or confocal laser endomicroscopy (CLE), is a modern imaging technique that allows the examination of real-time microscopic and histological features inside the body. In the word "endomicroscopy", endo- means "within" and -skopein means "to view or observe".
Fluorescence and confocal microscopes operating principle. Confocal microscopy, most frequently confocal laser scanning microscopy (CLSM) or laser scanning confocal microscopy (LSCM), is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out-of-focus light in image formation. [1]
The first technology (used by Carl Zeiss PALM) cuts around the sample then collects it by a "catapulting" technology. The sample can be catapulted from a slide or special culture dish by a defocused U.V laser pulse which generates a photonic force to propel the material off the slide/dish, a technique sometimes called Laser Micro-dissection ...
First workshop of Carl Zeiss in the center of Jena, c. 1847 Carl Zeiss Jena (1910) One of the Stasi's cameras with the special SO-3.5.1 (5/17mm) lens developed by Carl Zeiss, a so-called "needle eye lens", for shooting through keyholes or holes down to 1 mm in diameter 2 historical lenses of Carl Zeiss, Nr. 145077 and Nr. 145078, Tessar 1:4,5 F=5,5cm DRP 142294 (produced before 1910) Carl ...
1846: Carl Zeiss founded Carl Zeiss AG, to mass-produce microscopes and other optical instruments. 1850s: John Leonard Riddell, Professor of Chemistry at Tulane University, invents the first practical binocular microscope. [13] 1863: Henry Clifton Sorby develops a metallurgical microscope to observe structure of meteorites.