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  2. Chlorophyllase - Wikipedia

    en.wikipedia.org/wiki/Chlorophyllase

    Temperature also affects chlorophyllase activity. Wheat chlorophyllase is active from 25 to 75 °C. The enzyme is inactivated at temperatures above 85 °C. Wheat chlorophyllase is stable 20 °C higher than other chlorophyllases. These other chlorophyllases can stay active at temperatures up to 55 °C. [11]

  3. Enzyme assay - Wikipedia

    en.wikipedia.org/wiki/Enzyme_assay

    Enzyme activity as given in katal generally refers to that of the assumed natural target substrate of the enzyme. Enzyme activity can also be given as that of certain standardized substrates, such as gelatin, then measured in gelatin digesting units (GDU), or milk proteins, then measured in milk clotting units (MCU). The units GDU and MCU are ...

  4. Enzyme - Wikipedia

    en.wikipedia.org/wiki/Enzyme

    Enzyme activity. An enzyme's name is often derived from its substrate or the chemical reaction it catalyzes, with the word ending in -ase. [1]: 8.1.3 Examples are lactase, alcohol dehydrogenase and DNA polymerase. Different enzymes that catalyze the same chemical reaction are called isozymes. [1]: 10.3

  5. Enzyme unit - Wikipedia

    en.wikipedia.org/wiki/Enzyme_unit

    The enzyme unit, or international unit for enzyme (symbol U, sometimes also IU) is a unit of enzyme's catalytic activity. [ 1 ] 1 U (μmol/min) is defined as the amount of the enzyme that catalyzes the conversion of one micro mole of substrate per minute under the specified conditions of the assay method .

  6. Acetyl-CoA C-acetyltransferase - Wikipedia

    en.wikipedia.org/wiki/Acetyl-CoA_C-acetyltransferase

    In enzymology, an acetyl-CoA C-acetyltransferase (EC 2.3.1.9) is an enzyme that catalyzes the chemical reaction. 2 acetyl-CoA CoA + acetoacetyl-CoA. Hence, this enzyme has one substrate, acetyl-CoA, and two products, CoA and acetoacetyl-CoA. Acetyl-CoA C-acetyltransferase belongs to the thiolase family of enzymes.

  7. Taq polymerase - Wikipedia

    en.wikipedia.org/wiki/Taq_polymerase

    At temperatures above 90 °C, Taq demonstrates very little or no activity at all, but the enzyme itself does not denature and remains intact. [5] Presence of certain ions in the reaction vessel also affects specific activity of the enzyme. Small amounts of potassium chloride (KCl) and magnesium ion (Mg 2+) promote Taq's enzymatic activity.

  8. Enzyme kinetics - Wikipedia

    en.wikipedia.org/wiki/Enzyme_kinetics

    Enzyme kinetics: behavior and analysis of rapid equilibrium and steady state enzyme systems. New York: Wiley. ISBN 978-0-471-30309-1. Advanced. Fersht A (1999). Structure and mechanism in protein science: a guide to enzyme catalysis and protein folding. San Francisco: W.H. Freeman. ISBN 978-0-7167-3268-6. Schnell S, Maini PK (2004).

  9. Active site - Wikipedia

    en.wikipedia.org/wiki/Active_site

    The active site is usually a groove or pocket of the enzyme which can be located in a deep tunnel within the enzyme, [3] or between the interfaces of multimeric enzymes. An active site can catalyse a reaction repeatedly as residues are not altered at the end of the reaction (they may change during the reaction, but are regenerated by the end ...