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Optical mapping [1] is a technique for constructing ordered, genome-wide, high-resolution restriction maps from single, stained molecules of DNA, called "optical maps". By mapping the location of restriction enzyme sites along the unknown DNA of an organism, the spectrum of resulting DNA fragments collectively serves as a unique "fingerprint" or "barcode" for that sequence.
RseqFlow is an RNA-Seq analysis pipeline which offers an express implementation of analysis steps for RNA sequencing datasets. It can perform pre and post mapping quality control (QC) for sequencing data, calculate expression levels for uniquely mapped reads, identify differentially expressed genes, and convert file formats for ease of ...
Flexible and fast read mapping program (twice as fast as BWA), achieves a mapping sensitivity comparable to Stampy. Internally uses a memory efficient index structure (hash table) to store positions of all 13-mers present in the reference genome. Mapping regions where pairwise alignments are required are dynamically determined for each read.
Sequence Alignment Map (SAM) is a text-based format originally for storing biological sequences aligned to a reference sequence developed by Heng Li and Bob Handsaker et al. [1] It was developed when the 1000 Genomes Project wanted to move away from the MAQ mapper format and decided to design a new format.
In 2004, Goldsmith suggested a "problem pyramid" of "six steps which must be performed in sequence": [6] Identify the real problem, opportunity or challenge; Identify the current measure(s) which show that the problem is real; Identify the goal measure(s) to show the problem has been addressed and the value of meeting it
The DNA sequencing is done on a chip that contains many ZMWs. Inside each ZMW, a single active DNA polymerase with a single molecule of single stranded DNA template is immobilized to the bottom through which light can penetrate and create a visualization chamber that allows monitoring of the activity of the DNA polymerase at a single molecule level.
Once the query sequence is uploaded/pasted into the search field, the user can select various parameters such as which species' genome to target (there are currently over 50 species available) and the assembly version of that genome (for example, the human genome has four assemblies to select from), the query type (i.e. whether the sequence ...
During sequencing, each base in the template is sequenced twice, and the resulting data are decoded according to this scheme. 2 Base Encoding , also called SOLiD ( sequencing by oligonucleotide ligation and detection ), is a next-generation sequencing technology developed by Applied Biosystems and has been commercially available since 2008.