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The tool consists of a thin handle with a loop about 5 mm wide or smaller at the end. It was originally made of twisted metal wire (such as platinum, tungsten or nichrome), but disposable molded plastic versions are now common. The size of the loop determines the volume of liquid an inoculation loop can transfer.
Nichrome wire loop: used to inoculate test samples into culture media for bacterial or fungal cultures, antibiograms, etc.; sterilized by flaming to red hot before use Petri dish/agar plate: to act as a supporting container to hold the culture medium in Platinum wire loop
Sterilization of an inoculation needle via alcohol burner. The inoculation needle is sterilized using the aseptic technique. [1] [2] [3] [7] An open flame from an incinerator, a bunsen burner, or an alcohol burner is used to flame along the tip and the length of the needle that is to be in contact with the inoculum (or the propagule).
Nichrome (also known as NiCr, nickel-chromium or chromium-nickel) is a family of alloys of nickel and chromium (and occasionally iron [1]) commonly used as resistance wire, heating elements in devices like toasters, electrical kettles and space heaters, in some dental restorations (fillings) and in a few other applications.
The inoculation loop is then dragged across the surface of the agar back and forth in a zigzag motion until approximately 30% of the plate has been covered. The loop then is re-sterilized and the plate is turned 90 degrees. Starting in the previously streaked section, the loop is dragged through it two to three times continuing the zigzag pattern.
Nichrome, a non-magnetic 80/20 alloy of nickel and chromium, is the most common resistance wire for heating purposes because it has a high resistivity and resistance to oxidation at high temperatures, up to 1,400 °C (2,550 °F). When used as a heating element, resistance wire is usually wound into coils.
A type of cable called litz wire (from the German Litzendraht, braided wire) is used to mitigate skin effect for frequencies of a few kilohertz to about one megahertz. It consists of a number of insulated wire strands woven together in a carefully designed pattern, so that the overall magnetic field acts equally on all the wires and causes the ...
Wet each disk with about four inoculating loops of deionized water. Use a loop to aseptically transfer a large mass of pure bacteria to the disk. Observe the disk for up to three minutes. If the area of inoculation turns dark-blue to maroon to almost black, then the result is positive.