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R2A agar (Reasoner's 2A agar) is a culture medium [1] developed to study bacteria which normally inhabit potable water. [2] These bacteria tend to be slow-growing species and would quickly be suppressed by faster-growing species on a richer culture medium.
Mueller Hinton agar is a type of growth medium used in microbiology to culture bacterial isolates and test their susceptibility to antibiotics. This medium was first developed in 1941 by John Howard Mueller and Jane Hinton , who were microbiologists working at Harvard University.
De Man–Rogosa–Sharpe agar, often abbreviated to MRS, is a selective culture medium designed to favour the luxuriant growth of Lactobacilli for lab study. Developed in 1960, this medium was named for its inventors, Johannes Cornelis de Man [ Wikidata ] , Morrison Rogosa [ Wikidata ] , and Margaret Elisabeth Sharpe [ Wikidata ] .
11.0 g Agar; Preparation: 1. Heat with frequent agitation and boil for 1 minute to completely dissolve. 2. Autoclave at 121 °C for 15 minutes. Cool to 50 °C. 3. Add 50 ml filter sterilized 10% lactose solution and mix well (the lactose can be exchanged to other carbohydrates e.g. glucose, resulting in GM17 medium)
However, once the agar has solidified, the pH does not appear to change with temperature but remains at 6.9. For preparation of BCYE + antibiotics, add membrane-filtered antibiotics and mix. For BCYE + albumin agar, dissolve the albumin in distilled water and filter sterilize before addition to the medium. Dispense 20 mL per 15 X 100-mm Petri dish.
An agar plate – an example of a bacterial growth medium*: Specifically, it is a streak plate; the orange lines and dots are formed by bacterial colonies.. A growth medium or culture medium is a solid, liquid, or semi-solid designed to support the growth of a population of microorganisms or cells via the process of cell proliferation [1] or small plants like the moss Physcomitrella patens. [2]
The pour plate technique is the typical technique used to prepare plate count agars. Here, the inoculum is added to the molten agar before pouring the plate. The molten agar is cooled to about 45 degrees Celsius and is poured using a sterile method into a petri dish containing a specific diluted sample.
Schädler agar is a nutrient-rich growth medium primarily used in microbiology for the cultivation of anaerobic bacteria. It was developed to support the growth of a wide variety of anaerobic organisms, providing a conducive environment for both fastidious and non-fastidious anaerobes . [ 1 ]