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2,4-Dinitrophenylhydrazine (2,4-DNPH or DNPH) is the organic compound C 6 H 3 (NO 2) 2 NHNH 2. DNPH is a red to orange solid. It is a substituted hydrazine.
Hydrazines (R 2 N−NR 2) are a class of chemical compounds with two nitrogen atoms linked via a covalent bond and which carry from one up to four alkyl or aryl substituents. Hydrazines can be considered as derivatives of the inorganic hydrazine (H 2 N−NH 2), in which one or more hydrogen atoms have been replaced by hydrocarbon groups. [1]
2,4-DNP can mean: 2,4-Dinitrophenol , a small organic molecule formerly marketed as a pharmaceutical "diet aid" 2,4-Dinitrophenylhydrazine , Brady's reagent, used in organic chemical analysis
Colorimetric analysis is a method of determining the concentration of a chemical element or chemical compound in a solution with the aid of a color reagent.It is applicable to both organic compounds and inorganic compounds and may be used with or without an enzymatic stage.
[3] Two intersecting lines are drawn on the surface of the cap, the first with aniline or aniline water, the second with an aqueous solution of 65% nitric acid. The test is considered positive when a bright orange color forms where the lines cross. [4] [5] [6] Agaricus placomyces and Agaricus xanthodermus produce false negative reactions. [4] [5]
The dilute nature of the DPPH radicals (one unpaired spin per 41 atoms) results in a relatively small linewidth (1.5–4.7 G). The linewidth may however increase if solvent molecules remain in the crystal and if measurements are performed with a high-frequency EPR setup (~200 GHz), where the slight g -anisotropy of DPPH becomes detectable.
[2] Since Perrin's contribution, the technique has grown from determining binding isotherms under heavily controlled parameters, to the study of antigen-antibody, small molecule-protein, and hormone-receptor binding interactions. [4] A fluorescence polarization immunoassay was first described and used in the 1960s.
Phenol in the Berthelot reagent can be replaced by a variety of phenolic reagents, the most common being sodium salicylate, which is significantly less toxic. [1] This has been used for blood urea nitrogen (BUN) determinations and commonly is used to determine water and soil total and ammonia-N. Replacement of phenol by 2-phenylphenol reduces interferences by a variety of soil and water ...
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