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The most common supravital stain is performed on reticulocytes using new methylene blue or brilliant cresyl blue, which makes it possible to see the reticulofilamentous pattern of ribosomes characteristically precipitated in these live immature red blood cells by the supravital stains.
Brilliant cresyl blue is a supravital stain used for counting reticulocytes. [2] It is classified as an oxazine dye . N95 dust masks , eye shields, and gloves must all be worn when handling the chemical.
Those stains excluded by the living cells but taken up by the already dead cells are called vital stains (e.g. trypan blue or propidium iodide for eukaryotic cells). Those that enter and stain living cells are called supravital stains (e.g. New Methylene Blue and brilliant cresyl blue for reticulocyte staining). However, these stains are ...
New methylene blue (also NMB) [clarify] is an organic compound of the thiazine class of heterocycles. It is used as a stain and as an antimicrobial agent. It is classified as an azine dye, and the chromophore is a cation, the anion is often unspecified.
The methylene blue value is defined as the number of milliliter's standard methylene value solution decolorized 0.1 g of activated carbon (dry basis). [65] Methylene blue value reflects the amount of clay minerals in aggregate samples. [ 66 ]
Brilliant blue FCF: Erioglaucine FD&C Blue No. 1 Acid blue 9 Food blue 2 42090 triarylethlamine 3844-45-9: Brilliant cresyl blue: Cresyl blue BBS Basic dye 51010 oxazin 81029-05-2: Brilliant green: Malachite green G Zeylonka Basic green 1 42040 triarylmethane 633-03-4: Bromsulfthalein: BSP triarylmethane 71-67-0: Bromocresol green: BCG ...
Methyl blue is a chemical compound with the molecular formula C 37 H 27 N 3 Na 2 O 9 S 3.It is used as a stain in histology, [1] and stains collagen blue in tissue sections. It can be used in some differential staining techniques such as Mallory's connective tissue stain and Gömöri trichrome stain, and can be used to mediate electron transfer in microbial fuel cells.
Brilliant cresyl blue; C. Cresyl violet; G. Gallocyanin; N. Nile blue; Nile red; R. Resazurin This page was last edited on 25 January 2022, at 13:24 (UTC). Text is ...