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The analytical profile index (API) was invented in the 1970s in the United States by Pierre Janin of Analytab Products Inc. [2] The API test system is currently manufactured by bioMérieux. [3] The API range introduced a standardized and miniaturized version of existing techniques, which were considered complicated to perform and difficult to read.
When P. vulgaris is tested using the API 20E identification system [1] it produces positive results for sulfur reduction, urease production, tryptophan deaminase production, indole production, sometimes positive gelatinase activity, and saccharose fermentation, and negative results for the remainder of the tests on the testing strip.
Examining colonial morphology is the first step in the identification of an unknown microbe. The systematic assessment of the colonies' appearance, focusing on aspects like size, shape, colour, opacity, and consistency, provides clues to the identity of the organism, allowing microbiologists to select appropriate tests to provide a definitive ...
Klebsiella aerogenes, [2] previously known as Enterobacter aerogenes, is a Gram-negative, oxidase-negative, catalase-positive, citrate-positive, indole-negative, rod-shaped bacterium. [3]
Diagnostic microbiology is the study of microbial identification. Since the discovery of the germ theory of disease , scientists have been finding ways to harvest specific organisms. Using methods such as differential media or genome sequencing , physicians and scientists can observe novel functions in organisms for more effective and accurate ...
The culture is then incubated, allowing for microbial growth. [2] Most Dip slides consist of 1 - 2 agars attached to a flexible plastic paddle, this allows full contact of the agar onto the desired area for testing. [4] Most Dipslides come in a circular clear shatterproof tube that can be inserted into a dip-slide incubator.
McFarland standards. No. 0.5, 1 and 2. In microbiology, McFarland standards are used as a reference to adjust the turbidity of bacterial suspensions so that the number of bacteria will be within a given range to standardize microbial testing.
Both Int and IHF bind to attP and form an intasome, a DNA-protein-complex designed for site-specific recombination of the phage and host DNA. The original B-O-B' sequence is changed by the integration to B-O-P'-phage DNA-P-O-B'. The phage DNA is now part of the host's genome. [19]