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A disulfide ensemble is a grouping of all disulfide species with the same number of disulfide bonds, and is usually denoted as the 1S ensemble, the 2S ensemble, etc. for disulfide species having one, two, etc. disulfide bonds. Thus, the (26–84) disulfide species belongs to the 1S ensemble, whereas the (26–84, 58–110) species belongs to ...
DsbA is a bacterial thiol disulfide oxidoreductase (TDOR). DsbA is a key component of the Dsb (disulfide bond) family of enzymes. DsbA catalyzes intrachain disulfide bond formation as peptides emerge into the cell's periplasm. [2] Structurally, DsbA contains a thioredoxin domain with an inserted helical domain of unknown function. [3]
It is also used in molecular biology as an oxidising agent, for example to oxidise free thiols to form disulfide bonds in proteins. References
The structure of hydrogen disulfide is similar to that of hydrogen peroxide, with C 2 point group symmetry. Both molecules are distinctly nonplanar. The dihedral angle between the H a −S−S and S−S−H b planes is 90.6°, compared with 111.5° in H 2 O 2. The H−S−S bond angle is 92°, close to 90° for unhybridized divalent sulfur. [1]
Protein disulfide-isomerase has two catalytic thioredoxin-like domains (active sites), each containing the canonical CGHC motif, and two non catalytic domains. [4] [5] [6] This structure is similar to the structure of enzymes responsible for oxidative folding in the intermembrane space of the mitochondria; an example of this is mitochondrial IMS import and assembly (Mia40), which has 2 ...
The formation of multiple native disulfides remains challenging of native peptide synthesis by solid-phase methods. Random chain combination typically results in several products with nonnative disulfide bonds. [41] Stepwise formation of disulfide bonds is typically the preferred method, and performed with thiol protecting groups. [42]
The Disulfide bond oxidoreductase D (DsbD) family is a member of the Lysine Exporter (LysE) Superfamily. [1] A representative list of proteins belonging to the DsbD family can be found in the Transporter Classification Base .
2-Mercaptoethanol (also β-mercaptoethanol, BME, 2BME, 2-ME or β-met) is the chemical compound with the formula HOCH 2 CH 2 SH. ME or βME, as it is commonly abbreviated, is used to reduce disulfide bonds and can act as a biological antioxidant by scavenging hydroxyl radicals (amongst others).